ArticlePlant cell, tissue and organ culture2020
A reformulation of Murashige and Skoog medium (WPBS medium) improves embryogenesis, morphogenesis and transformation efficiency in temperate and tropical grasses and cereals.
Article in Plant cell, tissue and organ culture, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Culture Medium Composition and Light Intensity Shape Alternative Morphogenic Pathways from the Mature Embryo Shoot Apex ofPlants (Basel, Switzerland) · 2026Article
- Article
- Transgenic ZmMYB167 Miscanthus sinensis with increased lignin to boost bioenergy generation for the bioeconomy.Biotechnology for biofuels and bioproducts · 2023Article
- Callus Induction from Diverse Explants and Genotypes Enables Robust Transformation of Perennial Ryegrass (Plants (Basel, Switzerland) · 2022Article
- Protocol development for somatic embryogenesis, SSR markers and genetic modification of Stipagrostis pennata (Trin.) De Winter.Plant methods · 2021Article
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Authors and funding
1 author.
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Abstract
Slow callus growth is a barrier to efficient genetic transformation in some gramineous species. A reformulation of Murashige and Skoog (MS) medium, with additional magnesium sulphate, potassium phosphate, copper sulphate, proline and glutamine, termed WPBS medium, has been developed which improves all aspects of in vitro culture when compared with MS based media. Embryogenic callus could be produced more rapidly from responsive genotypes of sixteen cereal, forage, model and energy grass species, whether using embryos, shoot tips or proliferated meristems as explants. Three species were not transformed due to contamination or unsuitable explant, but thirteen species were transformed using an identical
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