Evidence map›Paper›PMID 32307615›Full record

ArticleInflammation2020

Exosomes from Long Noncoding RNA-Gm37494-ADSCs Repair Spinal Cord Injury via Shifting Microglial M1/M2 Polarization.

Minghao Shao, Mingming Jin, Shun Xu, Chaojun Zheng, Wei Zhu, Xiaosheng Ma, Feizhou Lv

Abstract read
PubMed Publisher
In one paragraph

Article in Inflammation, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.

0numbers the graph read from it
0cells of the map it votes in
70citing papers in PubMed
5.8field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

70 citing papers in PubMed, 111 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Extracellular vesicle-based therapeutic strategies for spinal cord injury.Extracellular vesicles and circulating nucleic acids · 2026
    Review
  5. Review
  6. Review
  7. Review
  8. Review
  9. Review
  10. LncRNAs Orchestrating Neuroinflammation: A Comprehensive Review.Cellular and molecular neurobiology · 2025
    Review
  11. Review
  12. Review
  13. NcRNAs: a potential treatment for spinal cord injury.Frontiers in cellular neuroscience · 2025
    Review
  14. Review
  15. Review
  16. Review
  17. Article
  18. Review
  19. Review
  20. Article

10 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Minghao ShaoDepartment of Orthopedics, Huashan Hospital, Fudan University, Shanghai, 200040, China.
Mingming JinShanghai Key Laboratory of Molecular Imaging, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China.
Shun XuDepartment of Orthopedics, Huashan Hospital, Fudan University, Shanghai, 200040, China.
Chaojun ZhengDepartment of Orthopedics, Huashan Hospital, Fudan University, Shanghai, 200040, China.
Wei ZhuDepartment of Orthopedics, Huashan Hospital, Fudan University, Shanghai, 200040, China.
Xiaosheng MaDepartment of Orthopedics, Huashan Hospital, Fudan University, Shanghai, 200040, China.
Feizhou LvDepartment of Orthopedics, Huashan Hospital, Fudan University, Shanghai, 200040, China. lufeizhou@hotmail.com.
Fudan University · CNHuashan Hospital · CNShanghai University of Medicine and Health Sciences · CN

Funding

National Natural Science Foundation of China 81871552Science Foundation for Distinguished Young Scholars of China 81802145
6 · The paper itself

Abstract

Spinal cord injury (SCI) may lead to severe motor and sensory dysfunction, causing high mortality and disability rates. Adipose tissue-derived mesenchymal stem/stromal cells (ADSCs), especially hypoxia-pretreated ADSCs, represent an effective therapy for SCI by promoting the secretion of exosomes (Exos). Here, we investigated the therapeutic efficacy of exosomes secreted by ADSCs under hypoxia (HExos) and explored potential target molecules. We utilized nanoparticle tracking analysis, electron microscopy, qRT-PCR, and western blotting to analyze differences between HExos and Exos groups. The expression of long noncoding RNAs (lncRNAs) was examined by high-throughput sequencing. The therapeutic effects of different Exos treatments were compared in vitro and in an SCI model in vivo. The interaction between lncRNAs, microRNAs, and mRNA was examined by luciferase reporter experiments. We employed enzyme-linked immunosorbent assay and immunofluorescence to measure inflammatory factor expression and microglial polarization. The results showed that HExos was more effective than Exos for repairing SCI by suppressing inflammatory factor expression, promoting functional recovery, and shifting microglia from M1 to M2 polarization. High-throughput sequencing showed that LncGm37494 expression was significantly higher in HExos than Exos, and its upregulation promoted microglial M1/M2 polarization by inhibiting miR-130b-3p and promoting PPARγ expression, as shown by luciferase reporter experiments. Exos from lncGm37494 overexpressing ADSCs showed a similar therapeutic effect than HExos. The results indicated that HExos repair SCI by delivering lncGm37494, advising that lncGm3749 functions importantly in microenvironmental regulation and shows possibility for SCI treatments.

Indexed as

AnimalsCell LineCell PolarityCells, CulturedExosomesMesenchymal Stem CellsMiceMice, Inbred C57BLMicrogliaRNA, Long NoncodingSpinal Cord InjuriesThoracic VertebraeRNA, Long Noncodingadipose tissue-derived mesenchymal stem/stromal cells (ADSCs)lncGm37494microglialmiR-130b-3pspinal cord injury

Identifiers

PMID32307615
OpenAlexW3017034037

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.