Evidence map›Paper›PMID 32306469›Full record

ArticleThe FEBS journal2021

Enzymology of the pathway for ATP production by arginine breakdown.

Tjeerd Pols, Shubham Singh, Cecile Deelman-Driessen, Bauke F Gaastra, Bert Poolman

Open access · hybridAbstract read
In one paragraph

Article in The FEBS journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
1.1field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 35 citations in OpenAlex.

  1. Article
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  5. Review
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  8. Deciphering the metabolism ofApplied and environmental microbiology · 2024
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  11. Bottom-Up Synthetic Biology Using Cell-Free Protein Synthesis.Advances in biochemical engineering/biotechnology · 2023
    Review
  12. Article
  13. Biomolecules · 2022
    Review
  14. Article
  15. Neuroinflammation, Sleep, and Circadian Rhythms.Frontiers in cellular and infection microbiology · 2022
    Review
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Tjeerd PolsDepartment of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute & Zernike Institute for Advanced Materials, University of Groningen, The Netherlands.
Shubham SinghDepartment of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute & Zernike Institute for Advanced Materials, University of Groningen, The Netherlands.
Cecile Deelman-DriessenDepartment of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute & Zernike Institute for Advanced Materials, University of Groningen, The Netherlands.
Bauke F GaastraDepartment of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute & Zernike Institute for Advanced Materials, University of Groningen, The Netherlands.
Bert PoolmanDepartment of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute & Zernike Institute for Advanced Materials, University of Groningen, The Netherlands.ORCID 0000-0002-1455-531X
University of Groningen · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In cells, the breakdown of arginine to ornithine and ammonium ion plus carbon dioxide is coupled to the generation of metabolic energy in the form of ATP. The arginine breakdown pathway is minimally composed of arginine deiminase, ornithine transcarbamoylase, carbamate kinase, and an arginine/ornithine antiporter; ammonia and carbon dioxide most likely diffuse passively across the membrane. The genes for the enzymes and transporter have been cloned and expressed, and the proteins have been purified from Lactococcus lactis IL1403 and incorporated into lipid vesicles for sustained production of ATP. Here, we study the kinetic parameters and biochemical properties of the individual enzymes and the antiporter, and we determine how the physicochemical conditions, effector composition, and effector concentration affect the enzymes. We report the K

Indexed as

Adenosine TriphosphateAmino Acid Transport SystemsAmmoniaAntiportersArginineBacterial ProteinsCarbon DioxideEnergy MetabolismGene Expression Regulation, BacterialHydrolasesKineticsLactococcus lactisLiposomesOrnithineOrnithine CarbamoyltransferasePhosphatidylcholines1,2-dioleoyl-sn-glycero-3-phosphoglycerol1,2-oleoylphosphatidylcholineAdenosine TriphosphateAmino Acid Transport SystemsAmmoniaAntiportersArcD protein, BacteriaArgininearginine deiminaseBacterial Proteinscarbamate kinaseCarbon Dioxidedioleoyl phosphatidylethanolamineHydrolasesLiposomesOrnithineOrnithine CarbamoyltransferasePhosphatidylcholinesPhosphatidylethanolaminesPhosphatidylglycerolsPhosphotransferases (Carboxyl Group Acceptor)Recombinant Proteinsarginine deiminasearginine deiminase pathwayarginine/ornithine antiportercarbamate kinaseenzyme kineticsLactococcus lactisornithine transcarbamoylase

Identifiers

PMID32306469
PMCPMC7818446
OpenAlexW3016558552

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.