Evidence map›Paper›PMID 32306082›Full record

ArticleArchives of toxicology2020

Unique microRNA alterations in hepatocellular carcinomas arising either spontaneously or due to chronic exposure to Ginkgo biloba extract (GBE) in B6C3F1/N mice.

Haruhiro Yamashita, Sailesh Surapureddi, Ramesh C Kovi, Sachin Bhusari, Thai Vu Ton, Jian-Liang Li, Keith R Shockley, Shyamal D Peddada, Kevin E Gerrish, Cynthia V Rider and 3 more

Open access · greenAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Archives of toxicology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.2field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Haruhiro YamashitaCellular and Molecular Pathology Branch, DNTP, NIEHS, Research Triangle Park, NC, 27709, USA.
Sailesh SurapureddiSignal Transduction Laboratory, DIR, NIEHS, Research Triangle Park, NC, 27709, USA.
Ramesh C KoviCellular and Molecular Pathology Branch, DNTP, NIEHS, Research Triangle Park, NC, 27709, USA.
Sachin BhusariCellular and Molecular Pathology Branch, DNTP, NIEHS, Research Triangle Park, NC, 27709, USA.
Thai Vu TonCellular and Molecular Pathology Branch, DNTP, NIEHS, Research Triangle Park, NC, 27709, USA.
Jian-Liang LiIntegrative Bioinformatics Support Group, DIR, NIEHS, Research Triangle Park, NC, 27709, USA.
Keith R ShockleyBiostatistics and Computational Biology Branch, DIR, NIEHS, Research Triangle Park, NC, 27709, USA.
Shyamal D PeddadaBiostatistics and Computational Biology Branch, DIR, NIEHS, Research Triangle Park, NC, 27709, USA.
Kevin E GerrishMolecular Genomics Core Laboratory, DIR, NIEHS, Research Triangle Park, NC, 27709, USA.
Cynthia V RiderToxicology Branch, DNTP, NIEHS, Research Triangle Park, NC, 27709, USA.
Mark J HoenerhoffCellular and Molecular Pathology Branch, DNTP, NIEHS, Research Triangle Park, NC, 27709, USA.
Robert C SillsCellular and Molecular Pathology Branch, DNTP, NIEHS, Research Triangle Park, NC, 27709, USA.
Arun R PandiriCellular and Molecular Pathology Branch, DNTP, NIEHS, Research Triangle Park, NC, 27709, USA. arun.pandiri@nih.gov.
Research Triangle Park Foundation · USNational Institute of Environmental Health Sciences · USExperimental Pathology Laboratories · US

Funding

Gene Expression and MicroarraysZICES102445 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI GERRISH, KEVIN · 2009 to 2025
$31.6M
Molecular Pathogenesis of Rodent NeoplasiaZIAES103349 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI PANDIRI, ARUN KUMAR · 2020 to 2021
$5.4M
DNA Microarray Data AnalysisZIAES103166 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI SHOCKLEY, KEITH · 2013 to 2025
$1.3M
Intramural NIH HHS Z99 ES999999Intramural NIH HHS ZIA ES103349
6 · The paper itself

Abstract

Ginkgo biloba extract (GBE) is used in traditional Chinese medicine as a herbal supplement for improving memory. Exposure of B6C3F1/N mice to GBE in a 2-year National Toxicology Program (NTP) bioassay resulted in a dose-dependent increase in hepatocellular carcinomas (HCC). To identify key microRNAs that modulate GBE-induced hepatocarcinogenesis, we compared the global miRNA expression profiles in GBE-exposed HCC (GBE-HCC) and spontaneous HCC (SPNT-HCC) with age-matched vehicle control normal livers (CNTL) from B6C3F1/N mice. The number of differentially altered miRNAs in GBE-HCC and SPNT-HCC was 74 (52 up and 22 down) and 33 (15 up and 18 down), respectively. Among the uniquely differentially altered miRNAs in GBE-HCC, miR-31 and one of its predicted targets, Cdk1 were selected for functional validation. A potential miRNA response element (MRE) in the 3'-untranslated regions (3'-UTR) of Cdk1 mRNA was revealed by in silico analysis and confirmed by luciferase assays. In mouse hepatoma cell line HEPA-1 cells, we demonstrated an inverse correlation between miR-31 and CDK1 protein levels, but no change in Cdk1 mRNA levels, suggesting a post-transcriptional effect. Additionally, a set of miRNAs (miRs-411, 300, 127, 134, 409-3p, and 433-3p) that were altered in the GBE-HCCs were also altered in non-tumor liver samples from the 90-day GBE-exposed group compared to the vehicle control group, suggesting that some of these miRNAs could serve as potential biomarkers for GBE exposure or hepatocellular carcinogenesis. These data increase our understanding of miRNA-mediated epigenetic regulation of GBE-mediated hepatocellular carcinogenesis in B6C3F1/N mice.

Indexed as

Transcriptome3' Untranslated RegionsAnimalsBiomarkers, TumorCarcinoma, HepatocellularCDC2 Protein KinaseCell Line, TumorCell Transformation, NeoplasticEpigenesis, GeneticFemaleGene Expression ProfilingGene Expression Regulation, NeoplasticGinkgo bilobaGinkgo ExtractLiver NeoplasmsMale3' Untranslated RegionsBiomarkers, TumorCDC2 Protein KinaseCdk1 protein, ratGinkgo biloba extractGinkgo ExtractMicroRNAsPlant ExtractsBiomarkerCdk1EpigeneticGinkgo biloba extractHepatocellular carcinomaMicroRNAmiR-31

Identifiers

PMID32306082
PMCPMC8077180
OpenAlexW3017162969

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.