ArticleMolecular and cellular probes2020
Development of qPCR platform with probes for quantifying prevalent and biomedically relevant human gut microbial taxa.
Article in Molecular and cellular probes, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Changes in bacterial and metabolic profiles in breastfed infants during dietary transition to solids.Pediatric research · 2026Article
- Impact of lactic acid bacteria andVeterinary and animal science · 2026Article
- A Comparison of the Response of the Human Intestinal Microbiota to Probiotic and Nutritional Interventions In Vitro and In Vivo-A Case Study.Nutrients · 2025Article
- Gut microbiota and kidney function in autosomal dominant polycystic kidney disease participants in Cameroon: a cross-sectional study.BMC nephrology · 2025Article
- Quantitative and dynamic profiling of human gut core microbiota by real-time PCR.Applied microbiology and biotechnology · 2024Article
- Intestinal Biomarkers and Their Importance in Canine Enteropathies.Veterinary medicine international · 2024Review
- Tomato Plant Microbiota under Conventional and Organic Fertilization Regimes in a Soilless Culture System.Microorganisms · 2023Article
- Gender Influences Gut Microbiota among Patients with Irritable Bowel Syndrome.International journal of molecular sciences · 2023Article
- Human Adult Microbiota in a Static Colon Model: AhR Transcriptional Activity at the Crossroads of Host-Microbe Interaction.Foods (Basel, Switzerland) · 2022Article
- Analysis of the gut microbiome in dogs and cats.Veterinary clinical pathology · 2022Review
- A Comprehensive Evaluation ofPathogens (Basel, Switzerland) · 2021Article
- The gut reaction to couples' relationship troubles: A route to gut dysbiosis through changes in depressive symptoms.Psychoneuroendocrinology · 2021Article
- Gut microbiome signature of Viliuisk encephalomyelitis in Yakuts includes an increase in microbes linked to lean body mass and eating behaviour.Orphanet journal of rare diseases · 2020Article
- Serum and fecal profiles of aromatic microbial metabolites reflect gut microbiota disruption in critically ill patients: a prospective observational pilot study.Critical care (London, England) · 2020Observational
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nowadays the advent of innovative high-throughput sequencing allows obtaining high-quality microbiome profiling. However, PCR-based tests are still considered the "golden standard" for many clinical applications. Here, we designed a qPCR-based platform with fluorescent-labeled oligonucleotide probes for assessing human gut microbiome composition. The system allows conducting qualitative and semiquantitative analysis for 12 prokaryotic taxa that are prevalent in the human gut and associated with diseases, diet, age and other factors. The platform was validated by comparing microbiome profile data obtained with two different methods - the platform and high-throughput 16S rRNA sequencing - across 42 stool samples. The test can form the basis for precise and cost-efficient microbiome assay for large-scale surveys including clinical trials with interventions related to diet and disease risks.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.