ArticleAmerican journal of clinical pathology2020
Assessment of Specimen Pooling to Conserve SARS CoV-2 Testing Resources.
Article in American journal of clinical pathology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 176 papers, 1 of them a synthesis that pooled it.
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Who cites it
176 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Efficient high-risk human papillomavirus screening through pooled testing: a simulation study based on real-world data in low- and middle-income countries.Journal of global health · 2026Article
- Seroprevalence of Dirofilaria immitis in traveling dogs: implications for transboundary pathogen movement.Parasites & vectors · 2026Article
- Factors affecting detection and quantification of Schistosoma haematobium eggs in pooled urine samples.PLoS neglected tropical diseases · 2026Article
- Pathogens on High-Touch Surfaces in an Arid Megacity: A Longitudinal Molecular Surveillance Study.Microorganisms · 2026Article
- Sample pooling approaches simulated under resource scarcity, lapses in testing capacity, and rapid processing demands for surveillance testing: a data-driven performance comparison.BMC infectious diseases · 2026Article
- A pooled testing strategy for enterovirus D68 to facilitate local, resource-conserving surveillance.Diagnostic microbiology and infectious disease · 2025Article
- Optimizing surveillance: feasibility of pooled sample testing for bovine alphaherpesvirus 1 detection by real-time PCR.Virusdisease · 2025Article
- Implementation of pooled testing to increase access to routine viral load monitoring for people living with HIV on antiretroviral therapy.Scientific reports · 2025Article
- Bayesian Additive Regression Trees for Group Testing Data.Statistics in medicine · 2025Article
- Performance of Urine Reagent Test Strips in DetectingMicroorganisms · 2025Article
- Reagent efficiency and analytical sensitivity optimization for a reliable SARS-CoV-2 pool-based testing strategy.Heliyon · 2025Article
- Assessment of the Effective Sensitivity of SARS-CoV-2 Sample Pooling Based on a Large-Scale Screening Experience: Retrospective Analysis.JMIR public health and surveillance · 2024Article
- Regression analysis of group-tested current status data.Biometrika · 2024Article
- Clinical sensitivity and time-to-result of a cascaded pooled testing approach for assessing the prevalence and intensity of Schistosoma haematobium infection.PLoS neglected tropical diseases · 2024Article
- Strategies for Increasing the Throughput of Genetic Screening: Lessons Learned from the COVID-19 Pandemic within a University Community.Biotech (Basel (Switzerland)) · 2024Article
- Evaluations of modes of pooling specimens for COVID-19 screened by quantitative PCR and droplet digital PCR.Scientific reports · 2024Article
- Clinical performance of AI-integrated risk assessment pooling reveals cost savings even at high prevalence of COVID-19.Scientific reports · 2024Article
- Evaluating and optimizing Acid-pH and Direct Lysis RNA extraction for SARS-CoV-2 RNA detection in whole saliva.Scientific reports · 2024Article
- Bioluminescence imaging reveals enhanced SARS-CoV-2 clearance in mice with combinatorial regimens.iScience · 2024Article
116 more citing papers are in PubMed but not listed here.
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6 authors.
Funding
Abstract
objectivesTo establish the optimal parameters for group testing of pooled specimens for the detection of SARS-CoV-2.
methodsThe most efficient pool size was determined to be five specimens using a web-based application. From this analysis, 25 experimental pools were created using 50 µL from one SARS-CoV-2 positive nasopharyngeal specimen mixed with 4 negative patient specimens (50 µL each) for a total volume of 250 µL. Viral RNA was subsequently extracted from each pool and tested using the CDC SARS-CoV-2 RT-PCR assay. Positive pools were consequently split into individual specimens and tested by extraction and PCR. This method was also tested on an unselected group of 60 nasopharyngeal specimens grouped into 12 pools.
resultsAll 25 pools were positive with cycle threshold (Ct) values within 0 and 5.03 Ct of the original individual specimens. The analysis of 60 specimens determined that 2 pools were positive followed by identification of 2 individual specimens among the 60 tested. This testing was accomplished while using 22 extractions/PCR tests, a savings of 38 reactions.
conclusionsWhen the incidence rate of SARS-CoV-2 infection is 10% or less, group testing will result in the saving of reagents and personnel time with an overall increase in testing capability of at least 69%.
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