Evidence map›Paper›PMID 32303872›Full record

SynthesisAnnals of biomedical engineering2020

Generating Cell Type-Specific Protein Signatures from Non-symptomatic and Diseased Tissues.

Jessica S Sadick, Lorin A Crawford, Harry C Cramer, Christian Franck, Shane A Liddelow, Eric M Darling

Open access · greenAbstract readMeta-AnalysisSystematic Review
PubMed Full text at PMC Publisher
In one paragraph

Synthesis in Annals of biomedical engineering, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Jessica S SadickDepartment of Molecular Pharmacology, Physiology, and Biotechnology, Brown University, Providence, RI, 02912, USA.
Lorin A CrawfordDepartment of Biostatistics, Brown University, Providence, RI, 02912, USA.
Harry C CramerCenter for Biomedical Engineering, Brown University, Providence, RI, 02912, USA.
Christian FranckCenter for Biomedical Engineering, Brown University, Providence, RI, 02912, USA.
Shane A LiddelowNeuroscience Institute, NYU Langone Medical Center, New York, NY, 10016, USA.
Eric M DarlingDepartment of Molecular Pharmacology, Physiology, and Biotechnology, Brown University, Providence, RI, 02912, USA. Eric_Darling@brown.edu.ORCID http://orcid.org/0000-0002-3932-8623
Brown University · USUniversity of Wisconsin–Madison · USThe University of Melbourne · AU

Funding

PSYCHOSOCIAL COREP30AG008051 · NIA · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · PI SHAO, YONGZHAO · 1990 to 2019
$36.1M
Postdoctoral Research Training in Neurodegenerative Disorders and the Aging BrainT32AG052909 · NIA · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · PI Helen E Scharfman, THOMAS M WISNIEWSKI · 2017 to 2026
$2.5M
High-yield, lineage-specific enrichment of living mesenchymal stem cellsR01AR063642 · NIAMS · BROWN UNIVERSITY · PI DARLING, ERIC M · 2013 to 2018
$1.8M
NIAMS NIH HHS R01 AR063642NIAMS NIH HHS R01 AR063642)NIA NIH HHS P30 AG008051NIA NIH HHS T32 AG052909
6 · The paper itself

Abstract

Here we demonstrate a technique to generate proteomic signatures of specific cell types within heterogeneous populations. While our method is broadly applicable across biological systems, we have limited the current work to study neural cell types isolated from human, post-mortem Alzheimer's disease (AD) and aged-matched non-symptomatic (NS) brains. Motivating the need for this tool, we conducted an initial meta-analysis of current, human AD proteomics studies. While the results broadly corroborated major neurodegenerative disease hypotheses, cell type-specific predictions were limited. By adapting our Formaldehyde-fixed Intracellular Target-Sorted Antigen Retrieval (FITSAR) method for proteomics and applying this technique to characterize AD and NS brains, we generated enriched neuron and astrocyte proteomic profiles for a sample set of donors (available at www.fitsarpro.appspot.com ). Results showed the feasibility for using FITSAR to evaluate cell-type specific hypotheses. Our overall methodological approach provides an accessible platform to determine protein presence in specific cell types and emphasizes the need for protein-compatible techniques to resolve systems complicated by cellular heterogeneity.

Indexed as

ProteomicsAlzheimer DiseaseAstrocytesBrainNeuronsAlzheimer’s diseaseAstrocytesBrainCellular heterogeneityEnrichmentProteomicsSorting

Identifiers

PMID32303872
PMCPMC7416432
OpenAlexW3017053096

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.