Evidence map›Paper›PMID 32303780›Full record

ArticleInflammation research : official journal of the European Histamine Research Society ... [et al.]2020

CHRFAM7A reduces monocyte/macrophage migration and colony formation in vitro.

Theresa W Chan, Simone Langness, Olga Cohen, Brian P Eliceiri, Andrew Baird, Todd W Costantini

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Inflammation research : official journal of the European Histamine Research Society ... [et al.], 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.1field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 19 citations in OpenAlex.

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  7. CHRFAM7A expression in mice increases resiliency after injury.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Theresa W ChanDivision of Trauma, Surgical Critical Care, Burns and Acute Care Surgery, Department of Surgery, University of California San Diego School of Medicine, 200 W. Arbor Drive #8896, San Diego, CA, 92103, USA.
Simone LangnessDivision of Trauma, Surgical Critical Care, Burns and Acute Care Surgery, Department of Surgery, University of California San Diego School of Medicine, 200 W. Arbor Drive #8896, San Diego, CA, 92103, USA.
Olga CohenDivision of Trauma, Surgical Critical Care, Burns and Acute Care Surgery, Department of Surgery, University of California San Diego School of Medicine, 200 W. Arbor Drive #8896, San Diego, CA, 92103, USA.
Brian P EliceiriDivision of Trauma, Surgical Critical Care, Burns and Acute Care Surgery, Department of Surgery, University of California San Diego School of Medicine, 200 W. Arbor Drive #8896, San Diego, CA, 92103, USA.
Andrew BairdDivision of Trauma, Surgical Critical Care, Burns and Acute Care Surgery, Department of Surgery, University of California San Diego School of Medicine, 200 W. Arbor Drive #8896, San Diego, CA, 92103, USA.
Todd W CostantiniDivision of Trauma, Surgical Critical Care, Burns and Acute Care Surgery, Department of Surgery, University of California San Diego School of Medicine, 200 W. Arbor Drive #8896, San Diego, CA, 92103, USA. tcostantini@health.ucsd.edu.ORCID http://orcid.org/0000-0002-0215-5327
University of California System · USUC San Diego Health System · US

Funding

OTOLARYNGOLOGY TRAINING IN IMMUN., VIROL. &MOLEC. BIOL.T32DC000028 · NIDCD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI RYAN, ALLEN F. · 1989 to 2020
$2.1M
The Human-Specific Gene CHRFAM7A in LeukocytesR01GM121530 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI COSTANTINI, TODD W · 2017 to 2021
$1.6M
NIDCD NIH HHS 5T32DC000028-27NIGMS NIH HHS 5R01GM121530-03NIGMS NIH HHS R01 GM121530
6 · The paper itself

Abstract

OBJECTIVE AND

designCHRFAM7A is a unique human gene that encodes a dominant negative inhibitor of the α7 nicotinic acetylcholine receptor. We have recently shown that CHRFAM7A is expressed in human leukocytes, increases cel-cell adhesion, and regulates the expression of genes associated with leukocyte migration. MATERIAL: Human THP-1, RAW264.7 and HEK293 cells.

methodsCell migration, cell proliferation and colony formation in soft agar to compare the biological activity of vector vs. CHRFAM7A-transduced cells.

resultsWe show that gene delivery of CHRFAM7A into the THP-1 human monocytic cell line reduces cell migration, reduces chemotaxis to monocyte chemoattractant protein, and reduces colony formation in soft agar.

conclusionTaken together, the findings demonstrate that CHRFAM7A regulates the biological activity of monocytes/macrophages to migrate and undergo anchorage-independent growth in vitro.

Indexed as

alpha7 Nicotinic Acetylcholine ReceptorAnimalsCell AdhesionCell MovementCell ProliferationGene ExpressionGene Expression RegulationHEK293 CellsHumansLeukocytesMacrophagesMiceMonocytesRAW 264.7 CellsStem CellsTHP-1 Cellsalpha7 Nicotinic Acetylcholine ReceptorChrna7 protein, humanDup α7-nicotinic acetylcholine receptorHuman-specific genesMonocyte migrationMyeloid cell self-renewalα7-nicotinic acetylcholine receptor

Identifiers

PMID32303780
PMCPMC7263946
OpenAlexW3016840557

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.