ArticleParasites & vectors2020
The role of oxygen depletion and subsequent radioprotective effects during irradiation of mosquito pupae in water.
Article in Parasites & vectors, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.
- Relationships between water quality and mosquito presence and abundance: a systematic review and meta-analysis.Journal of medical entomology · 2024Pooled it
- Sterile Insect Technique: Investigating the Impact of Larval Diet and Ionizing Radiation on the Mosquito Bacterial Microbiota.Insects · 2026Article
- Effects of different X-ray irradiation dosages on Wolbachia trans-infected and uninfected Aedes aegypti mosquitoes.PLoS neglected tropical diseases · 2026Article
- Evaluating the biological quality of mass-reared Anopheles arabiensis males irradiated as pupae or as adults.Malaria journal · 2025Article
- Current status of the sterile insect technique for the suppression of mosquito populations on a global scale.Infectious diseases of poverty · 2024Review
- Upscaling irradiation protocols of Aedes albopictus pupae within an SIT program in Reunion Island.Scientific reports · 2024Article
- Mass irradiation of adult Aedes mosquitoes using a coolable 3D printed canister.Scientific reports · 2024Article
- Article
- Does severe hypoxia during irradiation of Aedes aegypti pupae improve sterile male performance?Parasites & vectors · 2022Article
- Assessment of Compaction, Temperature, and Duration Factors for Packaging and Transporting of Sterile MaleInsects · 2022Article
- Radiation dose-rate is a neglected critical parameter in dose-response of insects.Scientific reports · 2022Article
- Effects of Chilling and Anoxia on the Irradiation Dose-Response in AdultFrontiers in bioengineering and biotechnology · 2022Article
- Response of male adultFrontiers in bioengineering and biotechnology · 2022Article
- Review
- Sterile Insect Technique in an Integrated Vector Management Program against Tiger MosquitoInsects · 2021Article
- Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRadiation induced sterility is the basis of the Sterile Insect Technique, by which a target insect pest population is suppressed by releasing artificially reared sterile males of the pest species in overflooding numbers over a target site. In order for the sterile males to be of high biological quality, effective standard irradiation protocols are required. Following studies investigating the effects of mosquito pupae irradiation in water versus in air, there is a need to investigate the oxy-regulatory behavior of mosquito pupae in water to better understand the consequences of irradiation in hypoxic versus normoxic conditions.
methodsPupae of Aedes aegypti, Ae. albopictus, and Anopheles arabiensis were submerged in water inside air-tight 2 ml glass vials at a density of 100 pupae/ml and the dissolved oxygen (DO) levels in the water were measured and plotted over time. In addition, male pupae of Ae. aegypti (aged 40-44 h), Ae. albopictus (aged 40-44 h) and An. arabiensis (aged 20-24 h) were irradiated in a gammacell220 at increasing doses in either hypoxic (water with < 0.5% O
resultsAll three species depleted the water of DO to levels under 0.5% within 30 minutes, with An. arabiensis consuming oxygen the fastest at under 10 minutes. Following irradiation, the protective effect of hypoxia was observed across species and doses (P < 0.0001), increasing at higher doses. This effect was most pronounced in An. arabiensis.
conclusionsThe consumption of dissolved oxygen by pupae submerged in water was significantly different between species, indicating that their oxy-regulatory capacity seems to have possibly evolved according to their preferred breeding site characteristics. This needs to be considered when sterilizing male mosquitoes at pupal stage in water. Depending on species, their DO consumption rates and their density, irradiation doses needed to achieve full sterility may vary significantly. Further assessments are required to ascertain optimal conditions in terms of ambient atmosphere during pupal irradiation to produce competitive sterile males, and temperature and density dependent effects are expected.
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