ArticleJournal of neuroscience methods2020
Methods for mechanical delivery of viral vectors into rhesus monkey brain.
Article in Journal of neuroscience methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 54 citations in OpenAlex.
- Surgical protocol for precise and high-throughput viral injections in rhesus monkey brain.STAR protocols · 2026Article
- Retrograde transduction of dopaminergic cells in substantia nigra of the rhesus monkey.Scientific reports · 2026Article
- Targeted gene transfer into developmentally defined cell populations of the primate brain.Cell reports · 2026Article
- Retrograde transduction of dopaminergic cells in substantia nigra of rhesus monkey.bioRxiv : the preprint server for biology · 2026Article
- Discrete interneuron subsets participate in GluN1/GluN3A excitatory glycine receptor (eGlyR)-mediated regulation of hippocampal network activity throughout development and evolution.Research square · 2025Article
- Discrete interneuron subsets participate in GluN1/GluN3A excitatory glycine receptor (eGlyR)-mediated regulation of hippocampal network activity throughout development and evolution.bioRxiv : the preprint server for biology · 2025Article
- A novel and evolutionarily conserved inhibitory circuit selectively regulates dentate gyrus mossy cell function.Research square · 2025Article
- A novel, evolutionarily conserved inhibitory circuit selectively regulates dentate gyrus mossy cell function.bioRxiv : the preprint server for biology · 2025Article
- Divergent opioid-mediated suppression of inhibition between hippocampus and neocortex across species and development.Neuron · 2025Article
- An enhancer-AAV toolbox to target and manipulate distinct interneuron subtypes.bioRxiv : the preprint server for biology · 2025Article
- Local Regulation of Striatal Dopamine Release Shifts from Predominantly Cholinergic in Mice to GABAergic in Macaques.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Divergent opioid-mediated suppression of inhibition between hippocampus and neocortex across species and development.bioRxiv : the preprint server for biology · 2025Article
- Comparative Physiology and Morphology of BLA-Projecting NBM/SI Cholinergic Neurons in Mouse and Macaque.The Journal of comparative neurology · 2024Article
- Chemogenetic Tools and their Use in Studies of Neuropsychiatric Disorders.Physiological research · 2024Review
- Comparative physiology and morphology of BLA-projecting NBM/SI cholinergic neurons in mouse and macaque.Research square · 2024Article
- Evaluation of [Molecular therapy : the journal of the American Society of Gene Therapy · 2024Article
- An open-source MRI compatible frame for multimodal presurgical mapping in macaque and capuchin monkeys.Journal of neuroscience methods · 2024Article
- Perceptography unveils the causal contribution of inferior temporal cortex to visual perception.Nature communications · 2024Article
- Synthesis and preclinical evaluation of [Scientific reports · 2024Article
- Surgical Procedure for Implantation of Opto-Array in Nonhuman Primates.Current protocols · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 1 institution in 1 country.
Funding
Abstract
backgroundModern molecular tools make it possible to manipulate neural activity in a reversible and cell-type specific manner. For rhesus monkey research, molecular tools are generally introduced via viral vectors. New instruments designed specifically for use in monkey research are needed to enhance the efficiency and reliability of vector delivery. NEW
methodA suite of multi-channel injection devices was developed to permit efficient and uniform vector delivery to cortical regions of the monkey brain. Manganese was co-infused with virus to allow rapid post-surgical confirmation of targeting accuracy using MRI. A needle guide was designed to increase the accuracy of sub-cortical targeting using stereotaxic co-ordinates.
resultsThe multi-channel injection devices produced dense, uniform coverage of dorsal surface cortex, ventral surface cortex, and intra-sulcal cortex, respectively. Co-infusion of manganese with the viral vector allowed for immediate verification of injection accuracy. The needle guide improved accuracy of targeting sub-cortical structures by preventing needle deflection. COMPARISON WITH EXISTING METHOD(S): The current methods, hand-held injections or single slow mechanical injection, for surface cortex transduction do not, in our hands, produce the density and uniformity of coverage provided by the injector arrays and associated infusion protocol.
conclusionsThe efficiency and reliability of vector delivery has been considerably improved by the development of new methods and instruments. This development should facilitate the translation of chemo- and optogenetic studies performed in smaller animals to larger animals such as rhesus monkeys.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.