ArticleThe Journal of experimental medicine2020
Continuous mitotic activity of primitive hematopoietic stem cells in adult mice.
Article in The Journal of experimental medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Hematopoietic stem and progenitor cell hierarchy is established by thrombopoietin-driven neonatal hematopoiesis.Stem cell reports · 2026Article
- Accumulation of free nuclei denotes defective phagocytic capacity of macrophages and occurs after infection withFrontiers in immunology · 2025Article
- Lineage tracing of nuclei in skeletal myofibers uncovers distinct transcripts and interplay between myonuclear populations.Nature communications · 2024Article
- A latent Axin2NPJ Regenerative medicine · 2024Article
- CD38 promotes hematopoietic stem cell dormancy.PLoS biology · 2024Article
- Lineage tracing of newly accrued nuclei in skeletal myofibers uncovers distinct transcripts and interplay between nuclear populations.bioRxiv : the preprint server for biology · 2023Article
- Article
- Cell therapy in end-stage liver disease: replace and remodel.Stem cell research & therapy · 2023Review
- Visualizing Sphingosine-1-Phosphate Receptor 1(S1PCellular and molecular neurobiology · 2023Article
- CycleFlow simultaneously quantifies cell-cycle phase lengths and quiescenceCell reports methods · 2022Article
- Ontogeny of cellular organization and LGR5 expression in porcine cochlea revealed using tissue clearing and 3D imaging.iScience · 2022Article
- Fate mapping of hematopoietic stem cells reveals two pathways of native thrombopoiesis.Nature communications · 2022Article
- Article
- Aging and Clonal Behavior of Hematopoietic Stem Cells.International journal of molecular sciences · 2022Review
- Visualization of individual cell division history in complex tissues using iCOUNT.Cell stem cell · 2021Article
- Hematopoiesis during Ontogenesis, Adult Life, and Aging.International journal of molecular sciences · 2021Review
- Aging of the Hematopoietic Stem Cell Niche: New Tools to Answer an Old Question.Frontiers in immunology · 2021Review
- Article
- The Polycomb-associated factor PHF19 controls hematopoietic stem cell state and differentiation.Science advances · 2020Article
- Molecular Modulation of Fetal Liver Hematopoietic Stem Cell Mobilization into Fetal Bone Marrow in Mice.Stem cells international · 2020Review
Corrections and comments
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Authors and funding
16 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The proliferative activity of aging hematopoietic stem cells (HSCs) is controversially discussed. Inducible fluorescent histone 2B fusion protein (H2B-FP) transgenic mice are important tools for tracking the mitotic history of murine HSCs in label dilution experiments. A recent study proposed that primitive HSCs symmetrically divide only four times to then enter permanent quiescence. We observed that background fluorescence due to leaky H2B-FP expression, occurring in all H2B-FP transgenes independent of label induction, accumulated with age in HSCs with high repopulation potential. We argue that this background had been misinterpreted as stable retention of induced label. We found cell division-independent half-lives of H2B-FPs to be short, which had led to overestimation of HSC divisional activity. Our data do not support abrupt entry of HSCs into permanent quiescence or sudden loss of regeneration potential after four divisions, but show that primitive HSCs of adult mice continue to cycle rarely.
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