ArticlePLoS pathogens2020
Role of MPK4 in pathogen-associated molecular pattern-triggered alternative splicing in Arabidopsis.
Article in PLoS pathogens, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 64 citations in OpenAlex.
- A Meta-Analysis Reveals Opposite Effects of Biotic and Abiotic Stresses on Transcript Levels of Arabidopsis Intracellular Immune Receptor Genes.Frontiers in plant science · 2021Pooled it
- Pathogen Effector-Mediated Reprogramming of Plant Alternative Splicing: From Immune Regulation to Crop Disease Resistance.Plants (Basel, Switzerland) · 2026Review
- Differential splicing fine-tunes guard cell gene expression and is required for drought tolerance in Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2026Article
- Loss of LEFKOTHEA Leads to Global Transcriptional and Post-Transcriptional Changes in Gene Expression During Early Light Response.Plants (Basel, Switzerland) · 2026Article
- Alternative splicing: an underexplored layer in immune receptor regulation, systemic resistance and priming.Frontiers in plant science · 2026Article
- RNA-binding proteins orchestrating immunity in plants.The Plant journal : for cell and molecular biology · 2025Review
- At-RS31 orchestrates hierarchical cross-regulation of splicing factors and integrates alternative splicing with TOR-ABA pathways.The New phytologist · 2025Article
- Global profiling of CPL3-mediated alternative splicing reveals regulatory mechanisms of DGK5 in plant immunity and phosphatidic acid homeostasis.Genome biology · 2025Article
- APlants (Basel, Switzerland) · 2025Article
- Phosphorylation of PIP2;7 by CPK28 or Phytophthora kinase effectors dampens pattern-triggered immunity in Arabidopsis.Plant communications · 2025Article
- ZmHSFA2B self-regulatory loop is critical for heat tolerance in maize.Plant biotechnology journal · 2025Article
- Microbial-mediated induced resistance: interactive effects for improving crop health.Frontiers in microbiology · 2025Review
- The Negative Regulators of the Basal Defence WRKY7, WRKY11 and WRKY17 Modulate the Jasmonic Acid Pathway and an Alternative Splicing Regulatory Network in Response to Pseudomonas syringae in Arabidopsis thaliana.Molecular plant pathology · 2024Article
- Alternative splicing of a potato disease resistance gene maintains homeostasis between growth and immunity.The Plant cell · 2024Article
- The plant endomicrobiome: Structure and strategies to produce stress resilient future crop.Current research in microbial sciences · 2024Review
- Determination of Differential Alternative Splicing Under Stress Conditions.Methods in molecular biology (Clifton, N.J.) · 2024Article
- MDF is a conserved splicing factor and modulates cell division and stress response inLife science alliance · 2023Article
- What's new in protein kinase/phosphatase signalling in the control of plant immunity?Essays in biochemistry · 2022Review
- Analysis of the Arabidopsis coilin mutant reveals a positive role of AtCOILIN in plant immunity.Plant physiology · 2022Article
- Alternative splicing as a key player in the fine-tuning of the immunity response in Arabidopsis.Molecular plant pathology · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alternative splicing (AS) of pre-mRNAs in plants is an important mechanism of gene regulation in environmental stress tolerance but plant signals involved are essentially unknown. Pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) is mediated by mitogen-activated protein kinases and the majority of PTI defense genes are regulated by MPK3, MPK4 and MPK6. These responses have been mainly analyzed at the transcriptional level, however many splicing factors are direct targets of MAPKs. Here, we studied alternative splicing induced by the PAMP flagellin in Arabidopsis. We identified 506 PAMP-induced differentially alternatively spliced (DAS) genes. Importantly, of the 506 PAMP-induced DAS genes, only 89 overlap with the set of 1950 PAMP-induced differentially expressed genes (DEG), indicating that transcriptome analysis does not identify most DAS events. Global DAS analysis of mpk3, mpk4, and mpk6 mutants in the absence of PAMP treatment showed no major splicing changes. However, in contrast to MPK3 and MPK6, MPK4 was found to be a key regulator of PAMP-induced DAS events as the AS of a number of splicing factors and immunity-related protein kinases is affected, such as the calcium-dependent protein kinase CPK28, the cysteine-rich receptor like kinases CRK13 and CRK29 or the FLS2 co-receptor SERK4/BKK1. Although MPK4 is guarded by SUMM2 and consequently, the mpk4 dwarf and DEG phenotypes are suppressed in mpk4 summ2 mutants, MPK4-dependent DAS is not suppressed by SUMM2, supporting the notion that PAMP-triggered MPK4 activation mediates regulation of alternative splicing.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.