ArticlePloS one2020
Lateral flow immunoassay (LFIA) for the detection of lethal amatoxins from mushrooms.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 54 citations in OpenAlex.
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- Development of a Protein-Free Nucleic Acid Lateral Flow Assay forAnalytical chemistry · 2025Article
- A systematic review of sensors to combat crime and routes to further sensor development.Frontiers in chemistry · 2025Review
- Rapid analysis of amatoxins in human urine by means of affinity column chromatography and liquid chromatography-high-resolution tandem mass spectrometry.Scientific reports · 2024Article
- A label-free electrochemical immunosensor based on decorated cellulose nanofibrous membrane for point-of-care diagnosis of amanitin poisoningLab on a chip · 2023Article
- A Sensitive, Cell-Based Assay for Measuring Low-Level Biological Activity of α-Amanitin.International journal of molecular sciences · 2023Article
- Amanitins: The Most Poisonous Molecules of the Fungal World.Molecules (Basel, Switzerland) · 2023Review
- Smartphone-based paper microfluidic competitive immunoassay for the detection of α-amanitin from mushrooms.Mikrochimica acta · 2022Article
- Development of a Monoclonal Antibody and a Serodiagnostic Lateral-Flow Device Specific toJournal of fungi (Basel, Switzerland) · 2022Article
- Lateral flow immunoassay for small-molecules detection in phytoproducts: a review.Journal of natural medicines · 2022Review
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- Optimizing Effective Parameters to Enhance the Sensitivity of Vertical Flow Assay for Detection ofBiosensors · 2022Article
- Utilizing the DNA Aptamer to Determine Lethal α-Amanitin in Mushroom Samples and Urine by Magnetic Bead-ELISA (MELISA).Molecules (Basel, Switzerland) · 2022Article
- Ten Years of Lateral Flow Immunoassay Technique Applications: Trends, Challenges and Future Perspectives.Sensors (Basel, Switzerland) · 2021Review
- Building of an Internal Transcribed Spacer (ITS) Gene Dataset to Support the Italian Health Service in Mushroom Identification.Foods (Basel, Switzerland) · 2021Article
- Evaluation and Comparison of Serological Methods for COVID-19 Diagnosis.Frontiers in molecular biosciences · 2021Review
- Human Poisoning from Poisonous Higher Fungi: Focus on Analytical Toxicology and Case Reports in Forensic Toxicology.Pharmaceuticals (Basel, Switzerland) · 2020Review
- Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mushroom poison that causes the most deaths is the class of toxins known as amatoxins. Current methods to sensitively and selectively detect these toxins are limited by the need for expensive equipment, or they lack accuracy due to cross-reactivity with other chemicals found in mushrooms. In this work, we report the development of a competition-based lateral flow immunoassay (LFIA) for the rapid, portable, selective, and sensitive detection of amatoxins. Our assay clearly indicates the presence of 10 ng/mL of α-AMA or γ-AMA and the method including extraction and detection can be completed in approximately 10 minutes. The test can be easily read by eye and has a presumed shelf-life of at least 1 year. From testing 110 wild mushrooms, the LFIA identified 6 out of 6 species that were known to contain amatoxins. Other poisonous mushrooms known not to contain amatoxins tested negative by LFIA. This LFIA can be used to quickly identify amatoxin-containing mushrooms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.