ArticlePloS one2020
Recapitulation of the accessible interface of biopsy-derived canine intestinal organoids to study epithelial-luminal interactions.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 74 citations in OpenAlex.
- Animal organoids as models for integrated One Health research.One health (Amsterdam, Netherlands) · 2026Review
- New Frontiers of Drug Development Through the Use of New Approach Methodologies.The AAPS journal · 2026Review
- Adult canine pancreatic organoids enable functional analysis of pancreatic epithelial barrier and inflammatory responses.BMC molecular and cell biology · 2026Article
- Canine duodenal organoids as a functional platform for intestinal CYP regulation and drug metabolism studies.Drug metabolism and disposition: the biological fate of chemicals · 2025Article
- A microfluidic platform for culturing and high-content imaging of adult stem cell-derived organoids.Scientific reports · 2025Article
- Structure of the Secretory Compartments in Goblet Cells in the Colon and Small Intestine.Cells · 2025Article
- From in vitro development to accessible luminal interface of neonatal bovine-derived intestinal organoids.BMC veterinary research · 2025Article
- Canine intestinal organoids as a platform for studying MHC class II expression in epithelial cells.BMC molecular and cell biology · 2025Article
- Development and characterization of a 2D porcine colonic organoid model for studying intestinal physiology and barrier function.PloS one · 2025Article
- Canine organoids: state-of-the-art, translation potential for human medicine and plea for standardization.Frontiers in veterinary science · 2025Review
- EnterohemorrhagicMicrobiology spectrum · 2024Article
- Proinflammatory cytokines suppress stemness-related properties and expression of tight junction in canine intestinal organoids.In vitro cellular & developmental biology. Animal · 2024Article
- Assessment of P-glycoprotein function using canine intestinal organoid-derived epithelial interfaces.Xenobiotica; the fate of foreign compounds in biological systems · 2024Article
- Pathogen-epithelium interactions and inflammatory responses in Salmonella Dublin infections using ileal monolayer models derived from adult bovine organoids.Scientific reports · 2024Article
- Assessment of cytochrome P450 induction in canine intestinal organoid models.Xenobiotica; the fate of foreign compounds in biological systems · 2024Article
- High-fat diet enhances cell proliferation and compromises intestinal permeability in a translational canine intestinal organoid model.BMC molecular and cell biology · 2024Article
- Differential Colonization and Mucus Ultrastructure Visualization in Bovine Ileal and Rectal Organoid-Derived Monolayers Exposed to EnterohemorrhagicInternational journal of molecular sciences · 2024Article
- Establishment and characterization of turtle liver organoids provides a potential model to decode their unique adaptations.Communications biology · 2024Article
- Three-Dimensional Morphogenesis in Canine Gut-on-a-Chip Using Intestinal Organoids Derived from Inflammatory Bowel Disease Patients.Journal of visualized experiments : JoVE · 2024Article
- Adult Animal Stem Cell-Derived Organoids in Biomedical Research and the One Health Paradigm.International journal of molecular sciences · 2024Review
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
Recent advances in canine intestinal organoids have expanded the option for building a better in vitro model to investigate translational science of intestinal physiology and pathology between humans and animals. However, the three-dimensional geometry and the enclosed lumen of canine intestinal organoids considerably hinder the access to the apical side of epithelium for investigating the nutrient and drug absorption, host-microbiome crosstalk, and pharmaceutical toxicity testing. Thus, the creation of a polarized epithelial interface accessible from apical or basolateral side is critical. Here, we demonstrated the generation of an intestinal epithelial monolayer using canine biopsy-derived colonic organoids (colonoids). We optimized the culture condition to form an intact monolayer of the canine colonic epithelium on a nanoporous membrane insert using the canine colonoids over 14 days. Transmission and scanning electron microscopy revealed a physiological brush border interface covered by the microvilli with glycocalyx, as well as the presence of mucin granules, tight junctions, and desmosomes. The population of stem cells as well as differentiated lineage-dependent epithelial cells were verified by immunofluorescence staining and RNA in situ hybridization. The polarized expression of P-glycoprotein efflux pump was confirmed at the apical membrane. Also, the epithelial monolayer formed tight- and adherence-junctional barrier within 4 days, where the transepithelial electrical resistance and apparent permeability were inversely correlated. Hence, we verified the stable creation, maintenance, differentiation, and physiological function of a canine intestinal epithelial barrier, which can be useful for pharmaceutical and biomedical researches.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.