Evidence map›Paper›PMID 32300977›Full record

ArticleJournal of fluorescence2020

Fluorescent Properties of Carboxyfluorescein Bifluorophores.

Vladimir A Povedailo, Ivan L Lysenko, Sergei A Tikhomirov, Dmitrii L Yakovlev, Dmitry A Tsybulsky, Aliaksandr S Kruhlik, Fan Fan, Yury V Martynenko-Makaev, Olga L Sharko, Pham V Duong and 2 more

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of fluorescence, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Fluorescent Sensing of SOSmall (Weinheim an der Bergstrasse, Germany) · 2025
    Article
  2. Article
  3. Molecular Beacon DNA Probes with Fluorescein Bifluorophore.Russian journal of bioorganic chemistry · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Vladimir A PovedailoB.I. Stepanov Institute of Physics, National Academy of Sciences of Belarus, 68 Nezavisimost' Ave, Minsk, 220072, Belarus.
Ivan L LysenkoInstitute of Physical Organic Chemistry, National Academy of Sciences of Belarus, 13 Surganova str, 220072, Minsk, Belarus.
Sergei A TikhomirovB.I. Stepanov Institute of Physics, National Academy of Sciences of Belarus, 68 Nezavisimost' Ave, Minsk, 220072, Belarus.
Dmitrii L YakovlevB.I. Stepanov Institute of Physics, National Academy of Sciences of Belarus, 68 Nezavisimost' Ave, Minsk, 220072, Belarus.
Dmitry A TsybulskyInstitute of Physical Organic Chemistry, National Academy of Sciences of Belarus, 13 Surganova str, 220072, Minsk, Belarus.
Aliaksandr S KruhlikInstitute of Physical Organic Chemistry, National Academy of Sciences of Belarus, 13 Surganova str, 220072, Minsk, Belarus.
Fan FanInstitute of Physical Organic Chemistry, National Academy of Sciences of Belarus, 13 Surganova str, 220072, Minsk, Belarus.
Yury V Martynenko-MakaevInstitute of Physical Organic Chemistry, National Academy of Sciences of Belarus, 13 Surganova str, 220072, Minsk, Belarus.
Olga L SharkoInstitute of Physical Organic Chemistry, National Academy of Sciences of Belarus, 13 Surganova str, 220072, Minsk, Belarus.
Pham V DuongInstitute of Physics, Vietnam Academy of Science and Technology, 10 Dao Tan, Ba Dinh, Hanoi, Vietnam.
Pham H MinhInstitute of Physics, Vietnam Academy of Science and Technology, 10 Dao Tan, Ba Dinh, Hanoi, Vietnam.
Vadim V ShmanaiInstitute of Physical Organic Chemistry, National Academy of Sciences of Belarus, 13 Surganova str, 220072, Minsk, Belarus. v.shmanai@gmail.com.ORCID http://orcid.org/0000-0003-1775-8101

Funding

Belarusian Republican Foundation for Fundamental Research F18V-010Vietnam Academy of Science and Technology (VN) QTBY01.07/18-19
6 · The paper itself

Abstract

Bright fluorescent probes with enhanced intensities in the fluorescein channel are of great value for plenty of biological applications. To design effective probes one should introduce as many as possible fluorophores to the biomolecule while leaving its native structure as intact as possible. To reach this compromise, we designed and synthesized fluorescein bifluorophores on the 3,5-diaminobenzoic acid scaffold, which allows for insertion of two fluorophores at one modification site of a biomolecule. Rigid structure of the branching linker group allows to minimize self-quenching the fluorophores. However, despite the structure similarities of fluorescein isomers (5-FAM and 6-FAM), different photophysical behavior was observed for the corresponding bifluorophores. Here we made efforts to get insight into these effects with the focus on the media viscosity impact.

Indexed as

CarboxyfluoresceinClick chemistryFluorescence intensityFluorescence lifetimeFluorescent antibody

Identifiers

PMID32300977

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.