ArticleMycorrhiza2020
HyLength: a semi-automated digital image analysis tool for measuring the length of roots and fungal hyphae of dense mycelia.
Article in Mycorrhiza, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Opening the black box: in situ imaging of arbuscular mycorrhizal fungal structures in soil using synchrotron-based micro-CT.The New phytologist · 2026Article
- Streamlining the isolation of fungal hyphae: a semi-automated approach for soil substrates.Journal of experimental botany · 2026Article
- Novel imaging approaches for visualizing root-mycorrhizal fungal interactions.Journal of experimental botany · 2026Review
- From cells to pixels: A decision tree for designing bioimage analysis pipelines.Journal of microscopy · 2025Article
- Exploring metal bioaccumulation ability of boreal white-rot fungi on fiberbank material.Bioengineered · 2025Article
- A travelling-wave strategy for plant-fungal trade.Nature · 2025Article
- Assembly and application of a low-cost high-resolution imaging device for hyphae in soil.PloS one · 2025Article
- Unlocking the biodegradative potential of native white-rot fungi: a comparative study of fiberbank organic pollutant mycoremediation.Bioengineered · 2024Article
- Phenotypic characteristics of the mycelium ofFrontiers in bioengineering and biotechnology · 2024Article
- RocTest: A standardized method to assess the performance of root organ cultures in the propagation of arbuscular mycorrhizal fungi.Frontiers in microbiology · 2022Article
- Direct transfer of zinc between plants is channelled by common mycorrhizal network of arbuscular mycorrhizal fungi and evidenced by changes in expression of zinc transporter genes in fungus and plant.Environmental microbiology · 2021Article
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6 authors.
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Abstract
In plant-fungus phenotyping, determining fungal hyphal and plant root lengths by digital image analysis can reduce labour and increase data reproducibility. However, the degree of software sophistication is often prohibitive and manual measuring is still used, despite being very time-consuming. We developed the HyLength tool for measuring the lengths of hyphae and roots in in vivo and in vitro systems. The HyLength was successfully validated against manual measures of roots and fungal hyphae obtained from all systems. Compared with manual methods, the HyLength underestimated Medicago sativa roots in the in vivo system and Rhizophagus irregularis hyphae in the in vitro system by about 12 cm per m and allowed to save about 1 h for a single experimental unit. As regards hyphae of R. irregularis in the in vivo system, the HyLength overestimated the length by about 21 cm per m compared with manual measures, but time saving was up to 20.5 h per single experimental unit. Finally, with hyphae of Aspergillus oryzae, the underestimation was about 8 cm per m with a time saving of about 10 min for a single germinating spore. By benchmarking the HyLength against the AnaMorf plugin of the ImageJ/Fiji, we found that the HyLength performed better for dense fungal hyphae, also strongly reducing the measuring time. The HyLength can allow measuring the length over a whole experimental unit, eliminating the error due to sub-area selection by the user and allowing processing a high number of samples. Therefore, we propose the HyLength as a useful freeware tool for measuring fungal hyphae of dense mycelia.
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