ArticleScience advances2020
High-resolution 4-D acquisition of freely swimming human sperm cells without staining.
Article in Science advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed.
- Clinical outcome using the Q300™ device in a reproductive laboratory environment: an open-label, non-controlled and non-randomized study.Reproductive biology and endocrinology : RB&E · 2025Trial
- Polarization Holographic 4D Phenotyping Reveals Nanoplastic Induced Dysfunction in Zooplankton.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Precise 3D Tracking of Highly Non-Planar Eukaryotic Flagellar Beating Patterns Using Digital Holographic Microscopy.Small methods · 2026Article
- Aging Remodels Mitochondrial Architecture Across the Male Reproductive Axis.Research square · 2026Article
- Label-Free Detection of Acrosome Reaction of Human Sperm Based on Diffraction Phase Microscopy.Chemical & biomedical imaging · 2026Article
- 3D+t human sperm flagellum centerline dataset.Scientific data · 2026Article
- Clinical Outcomes of ICSI Using Advanced Quantitative Phase Microscopy.Bioengineering (Basel, Switzerland) · 2026Article
- Quantitative holographic analysis in stallion spermatozoa following cryopreservation.Scientific reports · 2025Article
- Cell Viability, Drug Screening, and Mechanism Study Under Mild Phototherapy Integrated Chemotherapy (PIC).Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- 3D+t Multifocal Imaging Dataset of Human Sperm.Scientific data · 2025Article
- Characterization of a Single-Capture Bright-Field and Off-Axis Digital Holographic Microscope for Biological Applications.Sensors (Basel, Switzerland) · 2025Article
- Swimming by Spinning: Spinning-Top Type Rotations Regularize Sperm Swimming Into Persistently Progressive Paths in 3D.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Improving sperm selection strategies for assisted reproduction through closing the knowledge gap in sperm maturation mechanics.Human reproduction open · 2025Review
- First clinical pregnancy and delivery achieved after using a new 3D imaging technology for sperm selection: a case report.Frontiers in reproductive health · 2025Article
- The Synergic Effect of Tubal Endometriosis and Women's Aging on Fallopian Tube Function: Insights from a 3D Mechanical Model.Bioengineering (Basel, Switzerland) · 2024Article
- Quantitative phase imaging based on holography: trends and new perspectives.Light, science & applications · 2024Review
- High space-time bandwidth product imaging in low coherence quantitative phase microscopy.Scientific reports · 2024Article
- All-optical dual module platform for motility-based functional scrutiny of microencapsulated probiotic bacteria.Biomedical optics express · 2024Article
- Evaluation of women's aging influence on sperm passage inside the fallopian tube using 3D dynamic mechanical modeling.Frontiers in bioengineering and biotechnology · 2024Article
- Structural Analysis of Sperm Centrioles Using N-STORM.Methods in molecular biology (Clifton, N.J.) · 2024Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
We present a new acquisition method that enables high-resolution, fine-detail full reconstruction of the three-dimensional movement and structure of individual human sperm cells swimming freely. We achieve both retrieval of the three-dimensional refractive-index profile of the sperm head, revealing its fine internal organelles and time-varying orientation, and the detailed four-dimensional localization of the thin, highly-dynamic flagellum of the sperm cell. Live human sperm cells were acquired during free swim using a high-speed off-axis holographic system that does not require any moving elements or cell staining. The reconstruction is based solely on the natural movement of the sperm cell and a novel set of algorithms, enabling the detailed four-dimensional recovery. Using this refractive-index imaging approach, we believe that we have detected an area in the cell that is attributed to the centriole. This method has great potential for both biological assays and clinical use of intact sperm cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.