ArticleScientific reports2020
Development of a Nanoparticle-based Lateral Flow Strip Biosensor for Visual Detection of Whole Nervous Necrosis Virus Particles.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Review
- Deciphering European Sea Bass (International journal of molecular sciences · 2025Article
- Article
- Lateral flow immunoassay for simultaneous detection of C. difficile, MRSA, and K. pneumoniae.Mikrochimica acta · 2024Article
- Recent Advances in Lateral Flow Assays for Viral Protein Detection with Nanomaterial-Based Optical Sensors.Biosensors · 2024Review
- Pen direct writing of SERRS-based lateral flow assays for detection of penicillin G in milk.Nanoscale advances · 2024Article
- Evaluation of rapid detection methods for H5N1 virus using biosensors: An AI-based study.Bioinformation · 2024Article
- Nervous Necrosis Virus Modulation of European Sea Bass (International journal of molecular sciences · 2023Article
- Gold Nanotapes and Nanopinecones in a Quantitative Lateral Flow Assay for the Cancer Biomarker Carcinoembryonic Antigen.ACS applied nano materials · 2023Article
- Improvements to the Rapid Detection of the Marine Pathogenic Bacterium,Microorganisms · 2022Article
- Simple and rapid detection of ractopamine in pork with comparison of LSPR and LFIA sensors.Journal of food and drug analysis · 2022Article
- Trace Determination of Grouper Nervous Necrosis Virus in Contaminated Larvae and Pond Water Samples Using Label-Free Fiber Optic Nanoplasmonic Biosensor.Biosensors · 2022Article
- Low-cost, point-of-care biomarker quantification.Current opinion in biotechnology · 2022Review
- Development of a paper printed colorimetric sensor based on Cu-Curcumin nanoparticles for evolving point-of-care clinical diagnosis of sodium.Scientific reports · 2022Article
- Broad-Spectrum Theranostics and Biomedical Application of Functionalized Nanomaterials.Polymers · 2022Review
- Carbon dots for virus detection and therapy.Mikrochimica acta · 2021Review
- Camelid Single-Domain Antibodies for the Development of Potent Diagnosis Platforms.Molecular diagnosis & therapy · 2021Review
- Silver-Assembled Silica Nanoparticles in Lateral Flow Immunoassay for Visual Inspection of Prostate-Specific Antigen.Sensors (Basel, Switzerland) · 2021Article
- Disturbance of cellular homeostasis as a molecular risk evaluation of human endothelial cells exposed to nanoparticles.Scientific reports · 2021Article
- In-silico designing of epitope-based vaccine against the seven banded grouper nervous necrosis virus affecting fish species.Network modeling and analysis in health informatics and bioinformatics · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Effective analysis of pathogens causing human and veterinary diseases demands rapid, specific and sensitive detection methods which can be applied in research laboratory setups and in field for routine diagnosis. Paper lateral flow biosensors (LFBs) have been established as attractive tools for such analytical applications. In the present study a prototype LFB was designed for whole particles (virions) detection of nodavirus or fish nervous necrosis virus. Nodavirus is an important threat in the aquaculture industry, causing severe economic losses and environmental problems. The LFB was based on polyclonal antibodies conjugated on gold nanoparticles for signal visualization. Brain and retinas from fish samples were homogenized, centrifuged and the supernatant was directly applied on the LFB. Formation of a red test line was indicative of nodavirus virions presence. Nodavirus visual detection was completed in short time (30 min). Key factors of the LFB development influencing the assays' detection limit were characterized and the optimum parameters were determined, enabling increased efficiency, excluding non-specific interactions. Therefore, the proposed LFB assay consists a robust, simple, low cost and accurate method for detection of nodavirus virions in fish samples. The proposed biosensor is ideal for development of a commercial kit to be used on aquaculture facilities by fish farmers. It is anticipated that disease monitoring and environmental safety will benefit from the simplification of time consuming and costly procedures.
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