ArticleProceedings of the National Academy of Sciences of the United States of America2020
A viral toolkit for recording transcription factor-DNA interactions in live mouse tissues.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 25 citations in OpenAlex.
- Article
- Antecedent enhancer activity predicts future susceptibility to seizures in mice.Nature communications · 2026Article
- Inducible Calling Cards: Developing Mouse Reagents for Experimentally Controlled Transposon InsertionbioRxiv : the preprint server for biology · 2025Article
- Single-cell technology grows up: Leveraging high-resolution omics approaches to understand neurodevelopmental disorders.Current opinion in neurobiology · 2025Review
- MYT1L deficiency impairs excitatory neuron trajectory during cortical development.Nature communications · 2024Article
- Pycallingcards: an integrated environment for visualizing, analyzing, and interpreting Calling Cards data.Bioinformatics (Oxford, England) · 2024Article
- A Cre-dependent massively parallel reporter assay allows for cell-type specific assessment of the functional effects of non-coding elements in vivo.Communications biology · 2023Article
- Calling Cards: A Customizable Platform to Longitudinally Record Protein-DNA Interactions Over Time in Cells and Tissues.Current protocols · 2023Article
- Calling Cards: a customizable platform to longitudinally record protein-DNA interactions over time in cells and tissues.bioRxiv : the preprint server for biology · 2023Article
- MYT1L is required for suppressing earlier neuronal development programs in the adult mouse brain.Genome research · 2023Article
- Pivotal role of tissue-resident memory lymphocytes in the control of mucosal infections: can mucosal vaccination induce protective tissue-resident memory T and B cells?Frontiers in immunology · 2023Article
- Measuring transcription factor binding and gene expression using barcoded self-reporting transposon calling cards and transcriptomes.NAR genomics and bioinformatics · 2022Article
- MYT1L in the making: emerging insights on functions of a neurodevelopmental disorder gene.Translational psychiatry · 2022Review
- The precursors of CD8Nature reviews. Immunology · 2022Review
- The qBED track: a novel genome browser visualization for point processes.Bioinformatics (Oxford, England) · 2021Article
- Direct In Vitro Reprogramming of Astrocytes into Induced Neurons.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Article
- A viral toolkit for recording transcription factor-DNA interactions in live mouse tissues.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- To Seize or Not to Seize? That is the (Epigenetic) Question.Epilepsy currentsArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
16 authors at 1 institution in 1 country.
Funding
Abstract
Transcription factors (TFs) enact precise regulation of gene expression through site-specific, genome-wide binding. Common methods for TF-occupancy profiling, such as chromatin immunoprecipitation, are limited by requirement of TF-specific antibodies and provide only end-point snapshots of TF binding. Alternatively, TF-tagging techniques, in which a TF is fused to a DNA-modifying enzyme that marks TF-binding events across the genome as they occur, do not require TF-specific antibodies and offer the potential for unique applications, such as recording of TF occupancy over time and cell type specificity through conditional expression of the TF-enzyme fusion. Here, we create a viral toolkit for one such method, calling cards, and demonstrate that these reagents can be delivered to the live mouse brain and used to report TF occupancy. Further, we establish a Cre-dependent calling cards system and, in proof-of-principle experiments, show utility in defining cell type-specific TF profiles and recording and integrating TF-binding events across time. This versatile approach will enable unique studies of TF-mediated gene regulation in live animal models.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.