ArticleBMC developmental biology2020
A practical staging atlas to study embryonic development of Octopus vulgaris under controlled laboratory conditions.
Article in BMC developmental biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Charting a Sustainable Course:Microorganisms · 2026Article
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- The vertebrate yolk sac: evolutionary origins and current advances in in vitro models.Development (Cambridge, England) · 2026Review
- Camera-type eye specific visual ontogeny in squid (Sepioteuthis lessoniana).The Journal of experimental biology · 2026Article
- A non-invasive method to genotype cephalopod sex by quantitative PCR.bioRxiv : the preprint server for biology · 2025Article
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- The hard life of an octopus embryo is seen through gene expression, energy metabolism, and its ability to neutralize radical oxygen species.Scientific reports · 2024Article
- FELASA Working Group report: Capture and transport of live cephalopods - recommendations for scientific purposes.Laboratory animals · 2024Article
- A chromosome-level reference genome for the common octopus, Octopus vulgaris (Cuvier, 1797).G3 (Bethesda, Md.) · 2023Article
- Benzophenone-3 does not Cause Oxidative Stress or B-esterase Inhibition During Embryo Development of Octopus maya (Voss and Solís Ramírez, 1966).Bulletin of environmental contamination and toxicology · 2023Article
- Abyssal hydrothermal springs-Cryptic incubators for brooding octopus.Science advances · 2023Article
- Cell type diversity in a developing octopus brain.Nature communications · 2022Article
- MicroRNAs are deeply linked to the emergence of the complex octopus brain.Science advances · 2022Article
- First known observations of brooding, development, and hatching of fertilized eggs for the North Pacific bigeye octopus,Ecology and evolution · 2022Article
- Deciphering regeneration through non-model animals: A century of experiments on cephalopod mollusks and an outlook at the future.Frontiers in cell and developmental biology · 2022Review
- Optimization of Whole Mount RNA MultiplexedFrontiers in physiology · 2022Article
- Article
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Authors and funding
5 authors.
Funding
Abstract
backgroundOctopus vulgaris has been an iconic cephalopod species for neurobiology research as well as for cephalopod aquaculture. It is one of the most intelligent and well-studied invertebrates, possessing both long- and short-term memory and the striking ability to perform complex cognitive tasks. Nevertheless, how the common octopus developed these uncommon features remains enigmatic. O. vulgaris females spawn thousands of small eggs and remain with their clutch during their entire development, cleaning, venting and protecting the eggs. In fact, eggs incubated without females usually do not develop normally, mainly due to biological contamination (fungi, bacteria, etc.). This high level of parental care might have hampered laboratory research on the embryonic development of this intriguing cephalopod.
resultsHere, we present a completely parameter-controlled artificial seawater standalone egg incubation system that replaces maternal care and allows successful embryonic development of a small-egged octopus species until hatching in a laboratory environment. We also provide a practical and detailed staging atlas based on bright-field and light sheet fluorescence microscopy imaging for precise monitoring of embryonic development. The atlas has a comparative section to benchmark stages to the different scales published by Naef (1928), Arnold (1965) and Boletzky (2016). Finally, we provide methods to monitor health and wellbeing of embryos during organogenesis.
conclusionBesides introducing the study of O. vulgaris embryonic development to a wider community, this work can be a high-quality reference for comparative evolutionary developmental biology.
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