ArticleChemosphere2020
Dugesia japonica is the best suited of three planarian species for high-throughput toxicology screening.
Article in Chemosphere, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 41 citations in OpenAlex.
- Timing of exposure impacts how organophosphorus pesticides affect the developing brain in the planarian Dugesia japonica.Archives of toxicology · 2026Article
- Advances in ecotoxicological research using planarians: a comprehensive review.Environmental science and pollution research international · 2026Review
- Planarian behavioral screening is a useful invertebrate model for evaluating seizurogenic chemicals.Neurotoxicology · 2026Article
- Rapid behavioral screening in the planarianFrontiers in toxicology · 2026Article
- Red Midge Larvae Are an Invertebrate Alternative Diet to Beef Liver for Planarian Husbandry.Biomolecules · 2025Article
- Statistical analysis of multi-endpoint phenotypic screening increases sensitivity of planarian neurotoxicity testing.Toxicological sciences : an official journal of the Society of Toxicology · 2025Article
- Planarian behavioral screening is a useful invertebrate model for evaluating seizurogenic chemicals.bioRxiv : the preprint server for biology · 2025Article
- Article
- Planarian Mucus: A Novel Source of Pleiotropic Cytotoxic and Cytostatic Agents against Cancer Cells.Biomolecules · 2024Article
- How neurobehavior and brain development in alternative whole-organism models can contribute to prediction of developmental neurotoxicity.Neurotoxicology · 2024Review
- Behavioral and pharmacological characterization of planarian nociception.Frontiers in molecular neuroscience · 2024Article
- Comparative toxicity assessment of glyphosate and two commercial formulations in the planarianFrontiers in toxicology · 2023Article
- New Worm on the Block: Planarians in (Neuro)Toxicology.Current protocols · 2022Article
- Bioactivation and detoxification of organophosphorus pesticides in freshwater planarians shares similarities with humans.Archives of toxicology · 2022Article
- Head removal enhances planarian electrotaxis.The Journal of experimental biology · 2022Article
- Tannic Acid-Iron Complex-Based Nanoparticles as a Novel Tool against Oxidative Stress.ACS applied materials & interfaces · 2022Article
- Chemical Exposure-Induced Developmental Neurotoxicity in Head-Regenerating Schmidtea mediterranea.Toxicological sciences : an official journal of the Society of Toxicology · 2022Article
- Planarian nociception: Lessons from a scrunching flatworm.Frontiers in molecular neuroscience · 2022Review
- Genotoxicity Evaluation of Metformin in Freshwater PlanarianBioMed research international · 2022Article
- Differences in neurotoxic outcomes of organophosphorus pesticides revealedFrontiers in toxicology · 2022Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
High-throughput screening (HTS) using new approach methods is revolutionizing toxicology. Asexual freshwater planarians are a promising invertebrate model for neurotoxicity HTS because their diverse behaviors can be used as quantitative readouts of neuronal function. Currently, three planarian species are commonly used in toxicology research: Dugesia japonica, Schmidtea mediterranea, and Girardia tigrina. However, only D. japonica has been demonstrated to be suitable for HTS. Here, we assess the two other species for HTS suitability by direct comparison with D. japonica. Through quantitative assessments of morphology and multiple behaviors, we assayed the effects of 4 common solvents (DMSO, ethanol, methanol, ethyl acetate) and a negative control (sorbitol) on neurodevelopment. Each chemical was screened blind at 5 concentrations at two time points over a twelve-day period. We obtained two main results: First, G. tigrina and S. mediterranea planarians showed significantly reduced movement compared to D. japonica under HTS conditions, due to decreased health over time and lack of movement under red lighting, respectively. This made it difficult to obtain meaningful readouts from these species. Second, we observed species differences in sensitivity to the solvents, suggesting that care must be taken when extrapolating chemical effects across planarian species. Overall, our data show that D. japonica is best suited for behavioral HTS given the limitations of the other species. Standardizing which planarian species is used in neurotoxicity screening will facilitate data comparisons across research groups and accelerate the application of this promising invertebrate system for first-tier chemical HTS, helping streamline toxicology testing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.