ArticleAnnals of botany2020
Excess nitrate induces nodule greening and reduces transcript and protein expression levels of soybean leghaemoglobins.
Article in Annals of botany, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 39 citations in OpenAlex.
- Functional Characterization ofPlants (Basel, Switzerland) · 2026Article
- Natural Variation of NAR5 Determines Nitrogenase Activity and the Yield in Soybean.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Nutrient-dependent regulation of symbiotic nitrogen fixation in legumes.Horticulture research · 2025Article
- Optimal cultivation concentration of duckweed for pollutant removal from biogas slurry.Scientific reports · 2025Article
- A Study on the Effect of Indirect Nitrate Supply on the Nitrogen Fixation Capacity of Soybean Nodules.Plants (Basel, Switzerland) · 2024Article
- Interaction Between Nitric Oxide and Silicon on Leghaemoglobin and S-Nitrosothiol Levels in Soybean Nodules.Biomolecules · 2024Article
- Dynamics of hemoglobins during nodule development, nitrate response, and dark stress in Lotus japonicus.Journal of experimental botany · 2024Article
- The NAC transcription factors SNAP1/2/3/4 are central regulators mediating high nitrogen responses in mature nodules of soybean.Nature communications · 2023Article
- Transcriptomic analysis of humic acid in relieving the inhibitory effect of high nitrogen on soybean nodulation.Frontiers in plant science · 2023Article
- Shoot-to-root translocated GmNN1/FT2a triggers nodulation and regulates soybean nitrogen nutrition.PLoS biology · 2022Article
- GmTOC1b inhibits nodulation by repressingFrontiers in plant science · 2022Article
- Lineage-Specific Rewiring of Core Pathways Predating Innovation of Legume Nodules Shapes Symbiotic Efficiency.mSystems · 2021Article
- Nitrate Transport and Distribution in Soybean Plants With Dual-Root Systems.Frontiers in plant science · 2021Article
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4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsEfficient biological nitrogen fixation (BNF) requires leghaemoglobin (Lb) to modulate oxygen pressure in nodules. Excess N supply severely inhibits BNF through effects on Lb during nodulation. As yet, a systematic identification and characterization of Lb-encoding genes in soybean has not been reported.
methodsThe effects of N on BNF were studied in soybean plants inoculated with rhizobia and exposed to excess or low N availability in hydroponic cultures. To identify soybean Lb proteins, BLAST searches were performed on the Phytozome website. Bioinformatic analysis of identified GmLbs was then carried out to investigate gene structure, protein homology and phylogenetic relationships. Finally, quantitative real-time PCR was employed to analyse the expression patterns of soybean Lb genes in various tissues and in response to high N availability. KEY
resultsExcess N significantly accelerated nodule senescence and the production of green Lb in nodules. In total, seven haemoglobin (Hb) genes were identified from the soybean genome, with these Hb genes readily split into two distinct clades containing predominantly symbiosis-associated or non-symbiotic Hb members. Expression analysis revealed that all of the symbiosis-associated Lbs except GmLb5 were specifically expressed in nodules, while the non-symbiotic GmHbs, GmHb1 and GmHb2, were predominantly expressed in leaves and roots, respectively. Among identified GmLbs, GmLb1-4 are the major Lb genes acting in soybean nodulation, and each one is also significantly suppressed by exposure to excess N.
conclusionsTaken together, the results show that excess N inhibits BNF by reducing nodule formation, Lb concentration and nitrogenase activity. The characteristics of the entire Hb family were analysed, and we found that GmLb1-4 are closely associated with nodule development and N2 fixation. This works forms the basis for further investigations of the role of Lbs in soybean nodulation.
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