Evidence map›Paper›PMID 32297290›Full record

ArticleMolecular biology reports2020

A strategy based on Amplified Fragment Length Polymorphism (AFLP) for routine genotyping of nontuberculous mycobacteria at the clinical laboratory.

Sara Blanco-Conde, Carolina González-Cortés, Ramiro López-Medrano, Juan José Palacios-Gutiérrez, Cristina Diez-Tascón, Teresa Nebreda-Mayoral, María Josefa Sierra-García, Octavio Miguel Rivero-Lezcano

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In one paragraph

Article in Molecular biology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 1 country.

Sara Blanco-CondeServicio de Análisis Clínicos, Hospital de Palamós, Edificio Fleming, Carrer Hospital 36, 17230, Girona, Spain.
Carolina González-CortésUnidad de Investigación, Hospital Universitario de León, Altos de Nava, s/n, 24080, León, Spain.
Ramiro López-MedranoServicio de Microbiología Clínica, Hospital Universitario de León, Altos de Nava, s/n, 24080, León, Spain.
Juan José Palacios-GutiérrezUnidad de Referencia Regional de Micobacterias, Servicio de Microbiología, Hospital Universitario Central de Asturias, Avda. Roma s/n, 33011, Oviedo, Asturias, Spain.
Cristina Diez-TascónServicio de Anatomía Patológica, Hospital Universitario de León, Altos de Nava, s/n, 24080, León, Spain.
Teresa Nebreda-MayoralServicio de Microbiología, Hospital Clínico Universitario de Valladolid, C/ Ramon y Cajal 3, 47005, Valladolid, Spain.
María Josefa Sierra-GarcíaServicio de Análisis Clínicos, Hospital Universitario de León, Altos de Nava, s/n, 24080, León, Spain.
Octavio Miguel Rivero-LezcanoUnidad de Investigación, Hospital Universitario de León, Altos de Nava, s/n, 24080, León, Spain. omrivero@ibsal.es.ORCID http://orcid.org/0000-0002-8793-0731
Hospital de León · ESHospital Clínico Universitario de Valladolid · ESHospital de Palamós · ESHospital Universitario Central de Asturias · ESInstituto de Investigación Biomédica de Salamanca · ES

Funding

Consejeria de Sanidad, Junta de Castilla y León (ES) GRS 1225/A/16Instituto de Salud Carlos III PTA2015-11248-I
6 · The paper itself

Abstract

The increasing worldwide incidence of mycobacteriosis and the need to achieve improved clinical management makes nontuberculous mycobacteria (NTM) genotyping a useful tool. However, because of technical difficulties, medium size microbiology laboratories do not attempt to compare the genetic patterns that each of their isolates present. We have aimed to optimize a genotyping method with a reduced hands-on experimental time and that requires few technical resources. A strategy based on the Amplified Fragment Length Polymorphism (AFLP) methodology was developed using two rare-cutters enzymes (SacI and BglII). One out of seven primers was sequentially used in each amplification reaction that was analyzed by agarose gel electrophoresis. This approach makes it possible the timely genotyping of a moderate number of strains and its characterization without the need of image analysis software. We have genotyped 28 Mycobacterium intracellulare and 4 M. abscessus. Clinical researchers are encouraged to routinely genotype their NTM isolates.

Indexed as

Amplified Fragment Length Polymorphism AnalysisGenotypeGenotyping TechniquesHumansMycobacteriumNontuberculous MycobacteriaPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthCross-contaminationEpidemiologyGenotypingNontuberculous mycobacteriaNosocomial infectionTransmission

Identifiers

PMID32297290
OpenAlexW3016628083

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.