ArticleExtremophiles : life under extreme conditions2020
Essentiality of the glnA gene in Haloferax mediterranei: gene conversion and transcriptional analysis.
Article in Extremophiles : life under extreme conditions, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 23 citations in OpenAlex.
- Dynamic interaction between Escherichia coli enterotoxins and bacteriocins.The FEBS journal · 2026Article
- Red Mud Potentially Alleviates Ammonia Nitrogen Inhibition in Swine Manure Anaerobic Digestion by Enhancing Phage-Mediated Ammonia Assimilation.Microorganisms · 2025Article
- Identification and genomic analysis of a thermophilic bacterial strain that reduces ammonia loss from composting.Microbiology spectrum · 2024Article
- Transcriptomic profiling of haloarchaeal denitrification through RNA-Seq analysis.Applied and environmental microbiology · 2024Article
- Glutamine synthetase and GlnR regulate nitrogen metabolism inApplied and environmental microbiology · 2023Article
- Saline-Alkali Soil Property Improved by the Synergistic Effects ofInternational journal of molecular sciences · 2023Article
- Article
- Effect of pII key nitrogen regulatory gene on strain growth and butenyl-spinosyn biosynthesis in Saccharopolyspora pogona.Applied microbiology and biotechnology · 2022Article
- Novel Glutamate-Putrescine Ligase Activity inBiomolecules · 2021Article
- Analysis ofGenes · 2021Article
- Article
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Authors and funding
8 authors at 1 institution in 1 country.
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Abstract
Glutamine synthetase is an essential enzyme in ammonium assimilation and glutamine biosynthesis. The Haloferax mediterranei genome has two other glnA-type genes (glnA2 and glnA3) in addition to the glutamine synthetase gene glnA. To determine whether the glnA2 and glnA3 genes can replace glnA in nitrogen metabolism, we generated deletion mutants of glnA. The glnA deletion mutants could not be generated in a medium without glutamine, and thus, glnA is an essential gene in H. mediterranei. The glnA deletion mutant was achieved by adding 40 mM glutamine to the selective medium. This conditional HM26-ΔglnA mutant was characterised with different approaches in the presence of distinct nitrogen sources and nitrogen starvation. Transcriptomic analysis was performed to compare the expression profiles of the strains HM26-ΔglnA and HM26 under different growth conditions. The glnA deletion did not affect the expression of glnA2, glnA3 and nitrogen assimilation genes under nitrogen starvation. Moreover, the results showed that glnA, glnA2 and glnA3 were not expressed under the same conditions. These results indicated that glnA is an essential gene for H. mediterranei and, therefore, glnA2 and glnA3 cannot replace glnA in the conditions analysed.
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