ArticleScientific reports2020
Preserving Airway Smooth Muscle Contraction in Precision-Cut Lung Slices.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 28 citations in OpenAlex.
- Longitudinally cultured precision cut lung slices as a predictiveNanomedicine (London, England) · 2026Article
- β-Hydroxybutyrate attenuates bronchial smooth muscle proinflammatory cytokine production and contraction.American journal of physiology. Cell physiology · 2026Article
- 4D Time-lapse imaging of cell dynamics in human alveolar tissue by label-free higher harmonic generation microscopy.Journal of translational medicine · 2026Article
- Insights from precision-cut lung slices-investigating mechanisms and therapeutics for pulmonary hypertension.Respiratory research · 2025Review
- Degradation of IL-4Ralpha by Immunoproteasome: implication in airway type 2 inflammation and hyperresponsiveness.Frontiers in immunology · 2025Article
- Activation of STAT3-mediated ciliated cell survival protects against severe infection by respiratory syncytial virus.The Journal of clinical investigation · 2024Article
- The Fibrotic Phenotype of Human Precision-Cut Lung Slices Is Maintained after Cryopreservation.Toxics · 2024Article
- Precision cut lung slices: an integrated ex vivo model for studying lung physiology, pharmacology, disease pathogenesis and drug discovery.Respiratory research · 2024Review
- Precision-cut lung slices as anmBio · 2024Article
- Precision cut lung slices: an innovative tool for lung transplant research.Frontiers in immunology · 2024Review
- Lung Organotypic Slices Enable Rapid Quantification of Acute Radiotherapy Induced Toxicity.Cells · 2023Article
- Culture of vibrating microtome tissue slices as a 3D model in biomedical research.Journal of biological engineering · 2023Review
- Desert particulate matter from Afghanistan increases airway obstruction in human distal lungs exposed to type 2 cytokine IL-13.Frontiers in medicine · 2023Article
- Perspectives on precision cut lung slices-powerful tools for investigation of mechanisms and therapeutic targets in lung diseases.Frontiers in pharmacology · 2023Review
- Computational, Ex Vivo, and Tissue Engineering Techniques for Modeling Large Airways.Advances in experimental medicine and biology · 2023Article
- Airway smooth muscle in contractility and remodeling of asthma: potential drug target mechanisms.Expert opinion on therapeutic targets · 2023Article
- Application of Precision-Cut Lung Slices as an In Vitro Model for Research of Inflammatory Respiratory Diseases.Bioengineering (Basel, Switzerland) · 2022Review
- Functional changes in long-term incubated rat precision-cut lung slices.Respiratory research · 2022Article
- Utilizing the Precision-Cut Lung Slice to Study the Contractile Regulation of Airway and Intrapulmonary Arterial Smooth Muscle.Journal of visualized experiments : JoVE · 2022Article
- Airway smooth muscle function in asthma.Frontiers in physiology · 2022Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Precision-cut lung slices (PCLS) are ideal for measuring small airway contraction. However, these measurements are currently limited to acute exposure scenarios that typically last a few minutes to a few hours. Using an insulin-supplemented culture medium, we prolong the small airway contractility in mouse PCLS for up to two weeks. Compared to conventional culture medium, insulin-supplemented culture medium provides no additional benefit in preserving cellular viability or airway structure. However, it protects the airway smooth muscle (ASM) against a loss of smooth muscle myosin heavy chain (SMMHC) expression. We elucidate the significance of this new culture medium for chronic disease modeling of IL-13-induced airway hyper-responsiveness.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.