ArticleNature communications2020
Regulation of reactive oxygen species during plant immunity through phosphorylation and ubiquitination of RBOHD.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 143 papers.
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Who cites it
143 citing papers in PubMed, 300 citations in OpenAlex.
- Receptor-like cytoplasmic kinases VII subfamily orchestrates reactive oxygen species signaling in plant immunity.Plant signaling & behavior · 2026Review
- Identifying anthracnose resistance genes involving in ROS and JA accumulation.Plant signaling & behavior · 2026Article
- Insights Into the Role of Lysine Acetylation of Non-Histone Proteins in Plant Immunity.Plant, cell & environment · 2026Review
- Allelic variation of an LRR only protein confers dual pathogen resistance in Arabidopsis.Nature communications · 2026Article
- Phospho-ubiquitin relay: a modular switch for plant immune fine-tuning.Science China. Life sciences · 2026Article
- A rice calmodulin-like protein OsCML27 participates in drought tolerance by fine-tuning OsRbohB-mediated ROS signaling.Science advances · 2026Article
- Crosstalk and reciprocal regulation of phosphorylation and ubiquitination mediated by receptor-like kinases and U-box E3 ligases during plant growth and environmental stress responses.Plant communications · 2026Review
- The genome of Cynanchum thesioides (Freyn) K. Schum provides insights into its systematics and candidate genes potentially associated with drought tolerance as a xerophyte.BMC plant biology · 2026Article
- Transcriptome analysis and characterization of cathepsin B-like protein inMolecular breeding : new strategies in plant improvement · 2026Article
- Comparative transcriptomics and targeted metabolomics reveal mechanisms of leaf greenness retention during tobacco (Nicotiana tabacum L.) air-curing.BMC plant biology · 2026Article
- Effector-Mediated Spatial Reprogramming of Glycolate Oxidase Subverts Peroxisomal and Membrane-Associated ROS Defences.Plant biotechnology journal · 2026Article
- Cell-type-specific immune programs orchestrate spatial defense in the Arabidopsis leaf epidermis.Nature communications · 2026Article
- The glutathione-related metabolism and the AhGST23 gene mediate drought and salt stresses tolerance in peanut (Arachis hypogaea L.).BMC plant biology · 2026Article
- Stripe rust fungus subverts wheat phase separation-mediated immunity to promote infection.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Screening and Validation of Interacting Proteins of Receptor-like Cytoplasmic Kinase OsRLCK118 Involved in Rice Blast Resistance.Journal of fungi (Basel, Switzerland) · 2026Article
- Ubiquitin-Mediated Proteolysis as a Regulator of the Plant Defense-Growth Balance.Plants (Basel, Switzerland) · 2026Review
- The engineered Immunoprotein enhance broad-spectrum disease resistance in solanaceous crops.BMC plant biology · 2026Article
- Peroxidase Gene CaPOD49 Suppresses Chilli Veinal Mottle Virus Infection and Increases Oxidative Stress Tolerance in Chilli Pepper.Molecular plant pathology · 2026Article
- Motif-based substrate mapping of the receptor-like cytoplasmic kinase BIK1 reveals novel components and regulatory nodes of plant immunity.Nature plants · 2026Article
- Genomic and microecological insights into the biocontrol mechanisms ofFrontiers in microbiology · 2026Article
83 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
Production of reactive oxygen species (ROS) is critical for successful activation of immune responses against pathogen infection. The plant NADPH oxidase RBOHD is a primary player in ROS production during innate immunity. However, how RBOHD is negatively regulated remains elusive. Here we show that RBOHD is regulated by C-terminal phosphorylation and ubiquitination. Genetic and biochemical analyses reveal that the PBL13 receptor-like cytoplasmic kinase phosphorylates RBOHD's C-terminus and two phosphorylated residues (S862 and T912) affect RBOHD activity and stability, respectively. Using protein array technology, we identified an E3 ubiquitin ligase PIRE (PBL13 interacting RING domain E3 ligase) that interacts with both PBL13 and RBOHD. Mimicking phosphorylation of RBOHD (T912D) results in enhanced ubiquitination and decreased protein abundance. PIRE and PBL13 mutants display higher RBOHD protein accumulation, increased ROS production, and are more resistant to bacterial infection. Thus, our study reveals an intricate post-translational network that negatively regulates the abundance of a conserved NADPH oxidase.
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