Evidence map›Paper›PMID 32294096›Full record

ArticlePloS one2020

A framework for gene mapping in wheat demonstrated using the Yr7 yellow rust resistance gene.

Laura-Jayne Gardiner, Pauline Bansept-Basler, Mohamed El-Soda, Anthony Hall, Donal M O'Sullivan

Open access · goldAbstract readValidation Study
In one paragraph

Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
3.5field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 22 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 2 countries.

Laura-Jayne GardinerIBM Research, Warrington, England, United Kingdom.ORCID 0000-0002-9177-4452
Pauline Bansept-BaslerSyngenta, Ferme de Moyencourt, Orgerus, France.
Mohamed El-SodaDepartment of Genetics, Faculty of Agriculture, Cairo University, Giza, Egypt.ORCID 0000-0002-6930-5246
Anthony HallEarlham Institute, Norwich, England, United Kingdom.ORCID 0000-0002-1806-020X
Donal M O'SullivanSchool of Agriculture, Policy and Development, University of Reading, Reading, England, United Kingdom.ORCID 0000-0003-4889-056X
Cairo University · EGEarlham Institute · GBUniversity of East Anglia · GBUniversity of Reading · GB

Funding

Biotechnology and Biological Sciences Research Council BB/J002542/1Biotechnology and Biological Sciences Research Council BB/J0072607/1Biotechnology and Biological Sciences Research Council BB/P016855/1
6 · The paper itself

Abstract

We used three approaches to map the yellow rust resistance gene Yr7 and identify associated SNPs in wheat. First, we used a traditional QTL mapping approach using a double haploid (DH) population and mapped Yr7 to a low-recombination region of chromosome 2B. To fine map the QTL, we then used an association mapping panel. Both populations were SNP array genotyped allowing alignment of QTL and genome-wide association scans based on common segregating SNPs. Analysis of the association panel spanning the QTL interval, narrowed the interval down to a single haplotype block. Finally, we used mapping-by-sequencing of resistant and susceptible DH bulks to identify a candidate gene in the interval showing high homology to a previously suggested Yr7 candidate and to populate the Yr7 interval with a higher density of polymorphisms. We highlight the power of combining mapping-by-sequencing, delivering a complete list of gene-based segregating polymorphisms in the interval with the high recombination, low LD precision of the association mapping panel. Our mapping-by-sequencing methodology is applicable to any trait and our results validate the approach in wheat, where with a near complete reference genome sequence, we are able to define a small interval containing the causative gene.

Indexed as

BasidiomycotaChromosome MappingChromosomes, PlantDisease ResistanceGenome, PlantHaplotypesPlant DiseasesPolymorphism, Single NucleotideQuantitative Trait LociTriticum

Identifiers

PMID32294096
PMCPMC7159211
OpenAlexW3015818878

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.