ArticleEmerging infectious diseases2020
Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2.
Article in Emerging infectious diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
75 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Molecular diagnosis of COVID-19 in different biologic matrix, their diagnostic validity and clinical relevance: A systematic review.Life sciences · 2020Pooled it
- Engineering of novel DNA polymerase variants for single enzyme quantitative multiplex reverse transcription-PCR.Scientific reports · 2025Article
- Simultaneous and sensitive detection ofFrontiers in bioengineering and biotechnology · 2025Article
- Employing electrochemically derived pH gradients for Lab-on-PCB protein preconcentration devices.Microsystems & nanoengineering · 2024Article
- TREM2Nature communications · 2023Article
- SARS-CoV-2 genotyping and sequencing following a simple and economical RNA extraction and storage protocol.PloS one · 2023Article
- Screening and confirmation tests for SARS-CoV-2: benefits and drawbacks.Beni-Suef University journal of basic and applied sciences · 2023Review
- SARS-CoV-2 in municipal wastewater treatment plant, collection network, and hospital wastewater.Environmental science and pollution research international · 2022Article
- Rapid Detection and Characterization of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Omicron Variant in a Returning Traveler.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2022Article
- Limitations of Molecular and Antigen Test Performance for SARS-CoV-2 in Symptomatic and Asymptomatic COVID-19 Contacts.Journal of clinical microbiology · 2022Article
- Direct PCR with the CDC 2019 SARS-CoV-2 assay: optimization for limited-resource settings.Scientific reports · 2022Article
- Longitudinal Analysis of SARS-CoV-2 Vaccine Breakthrough Infections Reveals Limited Infectious Virus Shedding and Restricted Tissue Distribution.Open forum infectious diseases · 2022Article
- Sequential development of several RT-qPCR tests using LNA nucleotides and dual probe technology to differentiate SARS-CoV-2 from influenza A and B.Microbial biotechnology · 2022Article
- A modified vaccinia Ankara vaccine expressing spike and nucleocapsid protects rhesus macaques against SARS-CoV-2 Delta infection.Science immunology · 2022Article
- SARS-CoV-2 Molecular Diagnostics in China.Clinics in laboratory medicine · 2022Review
- Boosting SARS-CoV-2 detection combining pooling and multiplex strategies.Scientific reports · 2022Article
- Duplex One-Step RT-qPCR Assays for Simultaneous Detection of Genomic and Subgenomic RNAs of SARS-CoV-2 Variants.Viruses · 2022Article
- Daily longitudinal sampling of SARS-CoV-2 infection reveals substantial heterogeneity in infectiousness.Nature microbiology · 2022Article
- Establishment of an in-house real-time RT-PCR assay for the detection of severe acute respiratory syndrome coronavirus 2 using the first World Health Organization international standard in a resource-limited country.Journal of clinical laboratory analysis · 2022Article
- SARS-CoV-2 Variants in Paraguay: Detection and Surveillance with an Economical and Scalable Molecular Protocol.Viruses · 2022Article
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.