Evidence map›Paper›PMID 32293515›Full record

ArticleJournal of experimental & clinical cancer research : CR2020

Long non-coding RNA NCK1-AS1 promotes the tumorigenesis of glioma through sponging microRNA-138-2-3p and activating the TRIM24/Wnt/β-catenin axis.

Lifa Huang, Xu Li, Hui Ye, Yajun Liu, Xiaolong Liang, Chao Yang, Lin Hua, Zhaoxian Yan, Xin Zhang

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of experimental & clinical cancer research : CR, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
3.8field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 53 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Lifa HuangDepartment of Neurosurgery, Zhejiang Provincial Hospital of Traditional Chinese Medicine/The First Affiliated Hospital of Zhejiang Chinese Medical University, No. 54, Youdian Road, Shangcheng District, Hangzhou, Zhejiang, 310006, People's Republic of China.
Xu LiDepartment of Neurosurgery, Zhejiang Provincial Hospital of Traditional Chinese Medicine/The First Affiliated Hospital of Zhejiang Chinese Medical University, No. 54, Youdian Road, Shangcheng District, Hangzhou, Zhejiang, 310006, People's Republic of China.
Hui YeDepartment of Neurosurgery, Zhejiang Provincial Hospital of Traditional Chinese Medicine/The First Affiliated Hospital of Zhejiang Chinese Medical University, No. 54, Youdian Road, Shangcheng District, Hangzhou, Zhejiang, 310006, People's Republic of China.
Yajun LiuDepartment of Neurosurgery, Zhejiang Provincial Hospital of Traditional Chinese Medicine/The First Affiliated Hospital of Zhejiang Chinese Medical University, No. 54, Youdian Road, Shangcheng District, Hangzhou, Zhejiang, 310006, People's Republic of China.
Xiaolong LiangDepartment of Neurosurgery, Zhejiang Provincial Hospital of Traditional Chinese Medicine/The First Affiliated Hospital of Zhejiang Chinese Medical University, No. 54, Youdian Road, Shangcheng District, Hangzhou, Zhejiang, 310006, People's Republic of China.
Chao YangDepartment of Neurosurgery, Zhejiang Provincial Hospital of Traditional Chinese Medicine/The First Affiliated Hospital of Zhejiang Chinese Medical University, No. 54, Youdian Road, Shangcheng District, Hangzhou, Zhejiang, 310006, People's Republic of China.
Lin HuaThe First Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, People's Republic of China.
Zhaoxian YanThe First Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, People's Republic of China.
Xin ZhangDepartment of Neurosurgery, Zhejiang Provincial Hospital of Traditional Chinese Medicine/The First Affiliated Hospital of Zhejiang Chinese Medical University, No. 54, Youdian Road, Shangcheng District, Hangzhou, Zhejiang, 310006, People's Republic of China. Zhangxin10292@163.com.
Zhejiang Chinese Medical University · CN

Funding

National Natural Science Foundation of China 81873186
6 · The paper itself

Abstract

backgroundGlioma is a common brain malignancy with high mortality. The competing endogenous RNA (ceRNA) networks may play key roles in cancer progression. This study was conducted to probe the role of long noncoding RNA (lncRNA) NCK1-AS1 in glioma progression and the involved mechanisms.

methodsMicroarray analyses were performed to explore the lncRNAs/miRNAs/genes with differential expression in glioma. NCK1-AS1 levels in glioma tissues and normal brain tissues, and in glioma cell lines and normal human glial cells were identified. The interactions among NCK1-AS1, miR-138-2-3p and TRIM24 were validated through luciferase reporter, RNA immunoprecipitation and RNA pull-down assays. Gain- and loss-of functions of NCK1-AS1, miR-138-2-3p and TRIM24 were performed to identify their roles in the behaviors of glioma cells. The activity of the Wnt/β-catenin pathway was measured. In vivo experiments were performed as well.

resultsHigh expression of NCK1-AS1 was found in glioma tissues and cells, especially in U251 cells. Online predictions and the integrated experiments identified that NCK1-AS1 elevated the TRIM24 expression through sponging miR-138-2-3p, and further activated the Wnt/β-catenin pathway. Artificial silencing of NCK1-AS1 or up-regulation of miR-138-2-3p led to inhibited proliferation, invasion and migration but promoted cell apoptosis of U251 cells, while up-regulation of TRIM24 reversed these changes, and it activated the Wnt/β-catenin pathway. The in vitro results were reproduced in in vivo experiments.

conclusionsOur study suggested that NCK1-AS1 might elevate TRIM24 expression and further activate the Wnt/β-catenin pathway via acting as a ceRNA for miR-138-2-3p. Silencing of NCK1-AS1 might inhibit the progression of glioma.

Indexed as

Animalsbeta CateninCarcinogenesisCarrier ProteinsFemaleGliomaHumansMiceMice, NudeMicroRNAsRNA, Long NoncodingTransfectionbeta CateninCarrier ProteinsMicroRNAsMIRN138 microRNA, humanRNA, Long NoncodingTRIM24 protein, humanGliomaLong non-coding RNA NCK1-AS1microRNA-138-2-3pTRIM24Wnt/β-catenin signaling pathway

Identifiers

PMID32293515
PMCPMC7158134
OpenAlexW3016214864

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.