Evidence map›Paper›PMID 32293484›Full record

ArticleJournal of orthopaedic surgery and research2020

MicroRNA-505 is involved in the regulation of osteogenic differentiation of MC3T3-E1 cells partially by targeting RUNX2.

Weihua Li, Zongchao Chen, Chuanqi Cai, Gunjun Li, Xiao Wang, Zhenyu Shi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of orthopaedic surgery and research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.8field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 34 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Weihua LiDepartment of Orthopedics, Huaihe Hospital, Henan University, Kaifeng, 475000, China.
Zongchao ChenDepartment of Orthopedics, Huaihe Hospital, Henan University, Kaifeng, 475000, China.
Chuanqi CaiDepartment of Orthopedics, Huaihe Hospital, Henan University, Kaifeng, 475000, China.
Gunjun LiDepartment of Orthopedics, Huaihe Hospital, Henan University, Kaifeng, 475000, China.
Xiao WangDepartment of Orthopedics, Huaihe Hospital, Henan University, Kaifeng, 475000, China.
Zhenyu ShiHenan Medicial School, Henan University, Jinming Road, Kaifeng, 475004, China. jizhi19840612@163.com.
Henan University Huaihe Hospital and Huaihe Clinical Institute · CNHenan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveEvidence suggests that microRNAs (miRNAs) regulate the expression of genes involved in bone metabolism. This study aimed to investigate the role of miR-505 in the osteogenic differentiation of MC3T3-E1 cells.

methodsWe performed miRNA sequencing to identify differentially expressed miRNAs between MC3T3-E1 cells treated with osteogenic induction medium (OIM) and control cells. Bioinformatics analysis was performed by using the TargetScan and miRDB databases. The expression of miR-505 in MC3T3-E1 cells was detected during osteogenic differentiation. After transfection with miR-505 mimic or miR-505 inhibitor, MC3T3-E1 cells were induced to differentiate into osteoblasts, and the expression of osteogenic differentiation markers (Runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), osteopontin (OPN), osteocalcin (OCN), and osterix (OSX)) was detected.

resultsmiR-505 was the most downregulated miRNA among the differentially expressed miRNAs. The RUNX2 gene was identified as a potential target of miR-505 using the target prediction program. miR-505 expression was downregulated during osteogenic differentiation of MC3T3-E1 cells. The expression of osteogenic marker genes was inhibited in MC3T3-E1 cells after transfection with miR-505. However, the expression of osteogenic marker genes was upregulated after transfection with miR-505 inhibitor.

conclusionThis study is the first to report miR-505 could bind to the RUNX2 gene and thus regulate partly the dysfunction of osteoblasts differentiation, which is expected to be targets for the treatment of osteoporosis.

Indexed as

AnimalsCell DifferentiationCell LineComputational BiologyCore Binding Factor Alpha 1 SubunitMiceMicroRNAsOsteogenesisProtein BindingStem CellsCore Binding Factor Alpha 1 SubunitMicroRNAsMIRN505 microRNA, mouseRunx2 protein, mouseMC3T3-E1 cellsmiR-505miRNA sequenceOsteogenic differentiationRUNX2

Identifiers

PMID32293484
PMCPMC7160991
OpenAlexW3016084408

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.