ArticleBMC genomics2020
Identification and analysis of the FAD gene family in walnuts (Juglans regia L.) based on transcriptome data.
Article in BMC genomics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- Effect of High Temperature and Drought on the Fatty Acid Composition of Oil of Different Linseed Varieties and Its Association with Gene Expression.International journal of molecular sciences · 2026Article
- Comparative Study of Fatty Acid Desaturase (FAD) Members Reveals Their Differential Roles in Upland Cotton.Plants (Basel, Switzerland) · 2025Article
- Comprehensive Analysis ofInternational journal of molecular sciences · 2025Article
- Genome-wide characterization of FAD gene family in Xanthoceras sorbifolium Bunge and germplasm assessment.PloS one · 2025Article
- Genome-wide analysis of theFrontiers in plant science · 2025Article
- Identification and Analysis ofPlants (Basel, Switzerland) · 2024Article
- Primary metabolomics and transcriptomic techniques were used to explore the regulatory mechanisms that may influence the flavor characteristics of freshFrontiers in plant science · 2024Article
- The identification and expression analysis of walnut Acyl-ACP thioesterases.Frontiers in genetics · 2024Article
- Whole-genome and genome-wide association studies improve key agricultural traits of safflower for industrial and medicinal use.Horticulture research · 2023Article
- KeyInternational journal of molecular sciences · 2023Article
- The role of walnut bZIP genes in explant browning.BMC genomics · 2023Article
- Comparative transcriptomic and evolutionary analysis of FAD-like genes of Brassica species revealed their role in fatty acid biosynthesis and stress tolerance.BMC plant biology · 2023Article
- Physaria fendleri FAD3-1 overexpression increases ɑ-linolenic acid content in Camelina sativa seeds.Scientific reports · 2023Article
- Pan-genome and transcriptome analyses provide insights into genomic variation and differential gene expression profiles related to disease resistance and fatty acid biosynthesis in eastern black walnut (Horticulture research · 2023Article
- Metabolomics and Transcriptomics Provide Insights into Lipid Biosynthesis in the Embryos of Walnut (Plants (Basel, Switzerland) · 2023Article
- The chromosome-level genome ofFrontiers in plant science · 2023Article
- Genome-Wide Characterization and Expression Analysis of Fatty acid Desaturase Gene Family in Poplar.International journal of molecular sciences · 2022Article
- Genome-Wide Identification and Functional Differentiation of Fatty Acid Desaturase Genes inPlants (Basel, Switzerland) · 2022Article
- Comparative Analysis and Structural Modeling ofBiology · 2022Article
- In Silico Analysis of Fatty Acid Desaturases Structures inInternational journal of molecular sciences · 2021Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundWalnut kernels contain a large amount of unsaturated fatty acids, such as linoleic acid and linolenic acid, which are essential fatty acids for humans and have important effects on growth and health. The main function of fatty acid desaturase (FAD), which is widely distributed in organisms, is to remove hydrogen from carbon chains in the biosynthesis of unsaturated fatty acids to generate C=C bonds.
resultsBy performing a series of bioinformatics analysis, 24 members of the JrFAD gene family were identified from the genome database of walnut, and then compared with the homologous genes from Arabidopsis. Phylogenetic analysis showed that JrFADs were classified into four subfamilies: the SAD desaturase subfamily, Δ7/Δ9 desaturase subfamily, Δ12/ω-3 desaturase subfamily and "front-end" desaturase subfamily. Meanwhile, the expression of fatty acid synthesis genes in walnut kernels at different developmental stages was analysed by transcriptome sequencing, with expression of JrFAD3-1, which encodes an enzyme involved in linolenic acid synthesis, being particularly prominent. The relative expression level of JrFAD3-1 changed dramatically with the kernel development stages and exhibited a Bell-Shaped Curve. A significant positive correlation was observed between the expression of JrFAD3-1 during 70-100 DAF (Days after flowering) and the content of alpha-linolenic acid during 100-130 DAF, with a correlation coefficient of 0.991. Additionally, JrFAD3-1 was proved closely related to homologous genes in Betula pendula and Corylus heterophylla, indicating that the conserved structure of FADs is consistent with classical plant taxonomy.
conclusionTwenty-four members JrFADs in walnut were identified and classified into four subfamilies. JrFAD3-1 may play significant roles in the biosynthesis of polyunsaturated fatty acids in walnut.
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