Evidence map›Paper›PMID 32292820›Full record

ArticleOpen medicine (Warsaw, Poland)2020

Can Keratin Scaffolds be used for Creating Three-dimensional Cell Cultures?

Marta Bochynska-Czyz, Patrycja Redkiewicz, Hanna Kozlowska, Joanna Matalinska, Marek Konop, Piotr Kosson

Abstract read
In one paragraph

Article in Open medicine (Warsaw, Poland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Marta Bochynska-CzyzDepartment of Neuropeptides, Mossakowski Medical Research Centre Polish Academy of Science, 02-106 Warsaw, 5 Pawinskiego Street, Poland.
Patrycja RedkiewiczDepartment of Neuropeptides, Mossakowski Medical Research Centre Polish Academy of Science, 02-106 Warsaw, 5 Pawinskiego Street, Poland.
Hanna KozlowskaLaboratory of Advanced Microscopy Techniques, Mossakowski Medical Research Centre Polish Academy of Sciences, 02-106 Warsaw, 5 Pawinskiego Street, Poland.
Joanna MatalinskaDepartment of Neuropeptides, Mossakowski Medical Research Centre Polish Academy of Science, 02-106 Warsaw, 5 Pawinskiego Street, Poland.
Marek KonopDepartment of Neuropeptides, Mossakowski Medical Research Centre Polish Academy of Science, 02-106 Warsaw, 5 Pawinskiego Street, Poland.
Piotr KossonToxicology Research Laboratory, Mossakowski Medical Research Centre Polish Academy of Sciences, 02-106 Warsaw, 5 Pawinskiego Street, Poland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Three-dimensional (3D) cell cultures were created with the use of fur keratin associated proteins (F-KAPs) as scaffolds. The procedure of preparation F-KAP involves combinations of chemical activation and enzymatic digestion. The best result in porosity and heterogeneity of F-KAP surface was received during pepsin digestion. The F-KAP had a stable structure, no changes were observed after heat treatment, shaking and washing. The 0.15-0.5 mm fraction had positive effect for formation of 3D scaffolds and cell culturing. Living rat mesenchymal cells on the F-KAP with no abnormal morphology were observed by SEM during 32 days of cell culturing.

Indexed as

3D keratin scaffoldscell culturingfurKAP

Identifiers

PMID32292820
PMCPMC7147289

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.