ArticleFrontiers in microbiology2020
Identifying Metabolic Inhibitors to Reduce Bacterial Persistence.
Article in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
- The diverse phenotypic and mutational landscape induced by fluoroquinolone treatment.mSystems · 2025Article
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- UNRAVELING CRP/cAMP-MEDIATED METABOLIC REGULATION INbioRxiv : the preprint server for biology · 2025Article
- (p)ppGpp-mediated GTP homeostasis ensures survival and antibiotic tolerance of Staphylococcus aureus.Communications biology · 2025Article
- Unveiling the critical roles of cellular metabolism suppression in antibiotic tolerance.npj antimicrobials and resistance · 2024Article
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- Stimulating Transcription in Antibiotic-Tolerant Escherichia coli Sensitizes It to Fluoroquinolone and Nonfluoroquinolone Topoisomerase Inhibitors.Antimicrobial agents and chemotherapy · 2023Article
- Dysregulated NAD(H) homeostasis associated with ciprofloxacin tolerance inFrontiers in microbiology · 2023Article
- Proton Motive Force Inhibitors Are Detrimental to Methicillin-Resistant Staphylococcus aureus Strains.Microbiology spectrum · 2022Article
- Pleiotropic actions of phenothiazine drugs are detrimental to Gram-negative bacterial persister cells.Communications biology · 2022Article
- Combined utilization of metabolic inhibitors to prevent synergistic multi-species biofilm formation.AMB Express · 2022Article
- High-Throughput Screening of a Promoter Library Reveals New Persister Mechanisms in Escherichia Coli.Microbiology spectrum · 2022Article
- Article
- Single-cell Raman spectroscopy identifiesFrontiers in microbiology · 2022Article
- Cellular Self-Digestion and Persistence in Bacteria.Microorganisms · 2021Review
- Using membrane perturbing small molecules to target chronic persistent infections.RSC medicinal chemistry · 2021Review
- β-Lactams against the Fortress of the Gram-PositiveChemical reviews · 2021Review
- Monitoring Persister Resuscitation with Flow Cytometry.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Flow-cytometry analysis reveals persister resuscitation characteristics.BMC microbiology · 2020Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Bacterial persisters are rare phenotypic variants that are temporarily tolerant to high concentrations of antibiotics. We have previously discovered that stationary-phase-cell subpopulations exhibiting high redox activities were less capable of producing proteins and resuming growth upon their dilution into fresh media. The redox activities of these cells were maintained by endogenous protein and RNA degradation, resulting in self-inflicted damage that transiently repressed the cellular functions targeted by antibiotics. Here, we showed that pretreatment of stationary-phase cells with an ATP synthase inhibitor, chlorpromazine hydrochloride (CPZ), significantly reduced stationary-phase-redox activities and protein degradation, and yielded cells that were more susceptible to cell death when exposed to antibiotics in fresh media. Leveraging this knowledge, we developed an assay integrating a degradable fluorescent protein system and a small library, containing FDA-approved drugs and antibiotics, to detect medically relevant drugs that potentially target persister metabolism. We identified a subset of chemical inhibitors, including polymyxin B, poly-L-lysine and phenothiazine anti-psychotic drugs, that were able to reduce the persistence phenotype in
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.