Evidence map›Paper›PMID 32291741›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2020

High prevalence of subclinical paratuberculosis in buffaloes (Bubalus bubalis) in Maranhão, Brazil.

Helder de Moraes Pereira, Hamilton Pereira Santos, Emerson Antônio Araújo de Oliveira, Thais Bastos Rocha, Ítala Mayara Silva Araújo, Diego Moraes Soares, Felício Garino Junior, Pedro Paulo Feitosa de Albuquerque, Rinaldo Aparecido Mota

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.7field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Helder de Moraes PereiraClinics Department, State University of Maranhão, Cidade Universitária Paulo VI, Tirirical, P.O. BOX 09, São Luís, MA, 65055-970, Brazil. helderpereira@professor.uema.br.
Hamilton Pereira SantosDepartment of Pathology, State University of Maranhão, São Luís, MA, Brazil.
Emerson Antônio Araújo de OliveiraPost-graduate Program in Animal Science, State University of Maranhão, São Luís, MA, Brazil.
Thais Bastos RochaPost-graduate Program in Animal Science, State University of Maranhão, São Luís, MA, Brazil.
Ítala Mayara Silva AraújoPost-graduate Program in Animal Science, State University of Maranhão, São Luís, MA, Brazil.
Diego Moraes SoaresPost-graduate Program in Animal Science, State University of Maranhão, São Luís, MA, Brazil.
Felício Garino JuniorResearcher the Program in Animal Sanitary Defense, State University of Maranhão, São Luís, MA, Brazil.
Pedro Paulo Feitosa de AlbuquerqueDepartment of Veterinary Medicine, Rural Federal University of Pernambuco, Recife, PE, Brazil.
Rinaldo Aparecido MotaDepartment of Veterinary Medicine, Rural Federal University of Pernambuco, Recife, PE, Brazil.
Universidade Estadual do Maranhão · BRUniversidade Federal de Pernambuco · BRUniversidade Federal Rural de Pernambuco · BR

Funding

Fundação de Amparo à Pesquisa e ao Desenvolvimento Científico e Tecnológico do Maranhão Projeto de Pesquisa
6 · The paper itself

Abstract

Paratuberculosis is an infectious, chronic, and incurable disease that affects ruminants, causing enteritis and chronic granulomatous lymphadenitis, characterized by malabsorption syndrome, its agent is the Mycobacterium avium subsp. paratuberculosis (MAP). Thus, the objective of this work was to identify and characterize MAP in buffalo herds slaughtered in Baixada Maranhense region. Samples of intestines, mesenteric lymph nodes, and ileocecal valves were collected from 115 buffaloes slaughtered at Baixada Maranhense slaughterhouses to perform the diagnosis by histopathological examination using staining with Hematoxylin and Eosin (H&E) and Ziehl-Neelsen, bacterial isolation, and real-time PCR. In the histopathology by H&E staining, there was evidence suggestive of paratuberculosis in 30% (31/115) of the buffaloes. With Ziehl-Neelsen staining, acid-fast bacilli (AFB) were visualized in 27% (26/115) of the tissue samples analyzed. MAP was isolated in 4.3% (5/115) of the fecal samples subjected to bacterial culture. The samples inoculated in HEYM with mycobactin J produced colonies identified with MAP according to their own morphological characteristics such as round, white, smooth and slightly rough, alcohol-acid staining, and slow growth with 8 weeks of incubation and mycobactin dependence. The agent confirmation was performed in five bacterial isolates (4.3%) and 15 (13%) fragments of jejunum, ileum, and mesenteric lymph node by the IS900 real-time PCR technique. The results of the present study demonstrate the subclinical occurrence of paratuberculosis in flocks of buffalo slaughtered in slaughterhouses of Baixada Maranhense.

Indexed as

AnimalsBrazilBuffaloesCattleCattle DiseasesDNA, BacterialFecesMycobacterium avium subsp. paratuberculosisParatuberculosisPrevalenceReal-Time Polymerase Chain ReactionDNA, BacterialBuffaloDiarrheaMycobacterium avium subsp. paratuberculosisParatuberculosis

Identifiers

PMID32291741
PMCPMC7455677
OpenAlexW3015388220

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.