ArticleChinese chemical letters = Zhongguo hua xue kuai bao2019
CRISPR-Cas13a mediated nanosystem for attomolar detection of canine parvovirus type 2.
Article in Chinese chemical letters = Zhongguo hua xue kuai bao, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Innovations, Applications, and Future Trends in Veterinary Diagnostic Technologies.Transboundary and emerging diseases · 2026Review
- Rapid visual detection and differentiation of canine and feline parvovirus via a one-tube RPA-CRISPR/Cas13a assay.Frontiers in microbiology · 2025Article
- Overview of Recent Advances in Canine Parvovirus Research: Current Status and Future Perspectives.Microorganisms · 2024Review
- CRISPR/Cas system and its application in the diagnosis of animal infectious diseases.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024Review
- Research progress of severe acute respiratory syndrome coronavirus 2 on aerosol collection and detection.Chinese chemical letters = Zhongguo hua xue kuai bao · 2023Review
- Facile, ultrasensitive, and highly specific diagnosis of goose astrovirus via reverse transcription-enzymatic recombinase amplification coupled with a CRISPR-Cas12a system detection.Poultry science · 2022Article
- Article
- Biosafety materials: Ushering in a new era of infectious disease diagnosis and treatment with the CRISPR/Cas system.Biosafety and health · 2022Review
- Ionizable lipid-assisted efficient hepatic delivery of gene editing elements for oncotherapy.Bioactive materials · 2022Article
- CRISPR-Cas13a system: A novel tool for molecular diagnostics.Frontiers in microbiology · 2022Review
- Clustered Regularly Interspaced Short Palindromic Repeats-Associated Proteins13a combined with magnetic beads, chemiluminescence and reverse transcription-recombinase aided amplification for detection of avian influenza a (H7N9) virus.Frontiers in bioengineering and biotechnology · 2022Article
- Research progress on detection techniques for point-of-care testing of foodborne pathogens.Frontiers in bioengineering and biotechnology · 2022Review
- Development of a high specificity typing method for the detection of herpes simplex virus.Frontiers in bioengineering and biotechnology · 2022Article
- CRISPR-Cas13: A new technology for the rapid detection of pathogenic microorganisms.Frontiers in microbiology · 2022Review
- CRISPR-Cas based virus detection: Recent advances and perspectives.Biosensors & bioelectronics · 2021Review
- Potential of CRISPR/Cas system in the diagnosis of COVID-19 infection.Expert review of molecular diagnostics · 2021Review
- Advances and insights in the diagnosis of viral infections.Journal of nanobiotechnology · 2021Review
- Review
- Current methods and prospects of coronavirus detection.Talanta · 2021Review
- Next-generation pathogen diagnosis with CRISPR/Cas-based detection methods.Emerging microbes & infections · 2020Review
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Canine parvovirus type 2 (CPV-2) infection is the most lethal disease of dogs with higher mortality in puppies worldwide. In today's world, dogs are an integral part of our communities as well as dogs breeding and rearing has become a lucrative business. Therefore, a fast, accurate, portable, and cost-effective CPV-2 detection method with the ability for on-site detection is highly desired. In this study, we for the first time proposed a nanosystem for CPV-2 DNA detection with RNA-guided RNA endonuclease Cas13a, which upon activation results in collateral RNA degradation. We expressed LwCas13a in prokaryotic expression system and purified it through nickel column. Activity of Cas13a was verified by RNA-bound fluorescent group while using a quenched fluorescent probe as signals. Further Cas13a was combined with Recombinase polymerase amplification (RPA) and T7 transcription to establish molecular detection system termed specific high-sensitivity enzymatic reporter un-locking (SHERLOCK) for sensitive detection of CPV-2 DNA. This nanosystem can detect 100 amol/L CPV-2 DNA within 30 min. The proposed nanosystem exhibited high specificity when tested for CPV-2 and other dog viruses. This CRISPR-Cas13a mediated sensitive detection approach can be of formidable advantage during CPV-2 outbreaks because it is time-efficient, less laborious and does not involve the use of sophisticated instruments.
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