ArticleSensors and actuators. B, Chemical2018
CRISPR/dCas9-mediated biosensor for detection of tick-borne diseases.
Article in Sensors and actuators. B, Chemical, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- Research progress on emerging and important Tick-Borne pathogens.Frontiers in microbiology · 2026Review
- CRISPR-Cas-Based Diagnostics in Biomedicine: Principles, Applications, and Future Trajectories.Biosensors · 2025Review
- CRISPR/Cas Technology for the Diagnosis of Animal Infectious Diseases.Microorganisms · 2025Review
- Shining the Path of Precision Diagnostic: Advancements in Photonic Sensors for Liquid Biopsy.Biosensors · 2025Review
- Article
- Recent Advances in the CRISPR/Cas-Based Nucleic Acid Biosensor for Food Analysis: A Review.Foods (Basel, Switzerland) · 2024Review
- CRISPR for companion diagnostics in low-resource settings.Lab on a chip · 2024Review
- PCR Independent Strategy-Based Biosensors for RNA Detection.Biosensors · 2024Review
- Update on tick-borne pathogens detection methods within ticks.Current research in parasitology & vector-borne diseases · 2024Article
- CRISPR/Cas12a-Based Detection Platform for Early and Rapid Diagnosis of Scrub Typhus.Biosensors · 2023Article
- Amplification-Based CRISPR/Cas12a Biosensor Targeting theACS omega · 2023Article
- CRISPR-Cas-based techniques for pathogen detection: Retrospect, recent advances, and future perspectives.Journal of advanced research · 2023Review
- Biofunctionalization of Multiplexed Silicon Photonic Biosensors.Biosensors · 2022Review
- Application of the CRISPR/Cas System in Pathogen Detection: A Review.Molecules (Basel, Switzerland) · 2022Review
- Prospects of Biosensors Based on Functionalized and Nanostructured Solitary Materials: Detection of Viral Infections and Other Risks.ACS omega · 2022Review
- Next-Generation Diagnostic with CRISPR/Cas: Beyond Nucleic Acid Detection.International journal of molecular sciences · 2022Review
- Biosensors promising bio-device for pandemic screening "COVID-19".Microchemical journal : devoted to the application of microtechniques in all branches of science · 2021Review
- CRISPR-based detection of SARS-CoV-2: A review from sample to result.Biosensors & bioelectronics · 2021Review
- CRISPR: A new paradigm of theranostics.Nanomedicine : nanotechnology, biology, and medicine · 2021Review
- Current methods and prospects of coronavirus detection.Talanta · 2021Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid and highly sensitive detection of biomolecules is greatly needed for pathogen diagnosis in clinical samples, but the method needs to be significantly improved in terms of sensitivity and specificity for actual use in clinical settings. Here, we report the development of an improved molecular diagnostics tool that utilizes CRISPR/dCas9-mediated biosensor that couples a nuclease inactivated Cas9 (dCas9) and single microring resonator biosensor, enables label-free and real-time detection of pathogenic DNA and RNA. We addressed the clinical utility of this CRISPR/dCas9-mediated biosensor in tick-borne illnesses including scrub typhus (ST) and severe fever with thrombocytopenia syndrome (SFTS), whose clinical presentations are too similar to be easily differentiated. By using CRISPR/dCas9-mediated biosensor, we achieved single molecule sensitivity for the detection of ST (0.54 aM) and SFTS (0.63 aM); this detection sensitivity is 100 times more sensitive than that of RT-PCR assay. Finally, CRISPR/dCas9-mediated biosensor was able to clearly distinguish between ST and SFTS in serum samples within 20 min. We believe that CRISPR/dCas9-mediated biosensor will be useful for rapid and accurate molecular diagnostic tool that is suitable for immediate clinical applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.