ArticleNature communications2020
Synthetic biology based construction of biological activity-related library of fungal decalin-containing diterpenoid pyrones.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 106 citations in OpenAlex.
- A Sulfonamide Intermediate Elicits Therapeutic Effect Against Sepsis by Enhancing Neutrophil Maturation.Journal of cellular and molecular medicine · 2026Article
- Discovery of Pyranoquinolone Diterpenoids Biosynthesized through Oxidative Indole Rearrangement and Atypical Terpene Cyclization.Journal of the American Chemical Society · 2026Article
- A functional atlas of secondary metabolite biosynthetic gene clusters governing growth, stress adaptation, and pathogenicity in Fusarium graminearum.Crop health · 2026Article
- Genome mining-based discovery of an atypical fungal non-reducing polyketide synthase encoding dimeric alkylresorcinol biosynthesis.The Journal of antibiotics · 2026Article
- Lysine-Targeting Inhibitors of Amyloidogenic Protein Aggregation: A Promise for Neurodegenerative Proteinopathies.JACS Au · 2025Review
- Genome Mining-Based Discovery of Pyrano[2,3-Organic letters · 2025Article
- Synthetic Biology in Natural Product Biosynthesis.Chemical reviews · 2025Review
- Discovery and Theoretical Studies of Nonenzymatic Polyketide Dimerizations of Chaetophenols.Organic letters · 2025Article
- Revisiting the Baddeley Reaction: Access to Functionalized Decalins by Charge-Promoted Alkane Functionalization.Angewandte Chemie (International ed. in English) · 2025Article
- Actinomycetes - The Repertoire of Diverse Bioactive Chemical Molecules: From Structures to Antibiotics.Current topics in medicinal chemistry · 2025Review
- Identification and Confirmation of Virulence Factor Production fromJournal of fungi (Basel, Switzerland) · 2024Article
- Methyltransferase Domain-Focused Genome Mining for Fungal Polyketide Synthases.Small methods · 2024Article
- Semi-synthesis of a DNA-Tagged Polyketide-Peptide Hybrid Macrocycle Using a Biosynthetically Prepared Fungal Macrolide as a Synthetic Component.Organic letters · 2024Article
- Discovery of fungal onoceroid triterpenoids through domainless enzyme-targeted global genome mining.Nature communications · 2024Article
- Combinatorial biosynthesis for the engineering of novel fungal natural products.Communications chemistry · 2024Review
- Combinatorial optimization of gene expression through recombinase-mediated promoter and terminator shuffling in yeast.Nature communications · 2024Article
- Genomics-driven derivatization of the bioactive fungal sesterterpenoid variecolin: Creation of an unnatural analogue with improved anticancer properties.Acta pharmaceutica Sinica. B · 2024Article
- Recent developments in the engineered biosynthesis of fungal meroterpenoids.Beilstein journal of organic chemistry · 2024Review
- Biologically active secondary metabolites from white-rot fungi.Frontiers in chemistry · 2024Review
- Genome mining of labdane-related diterpenoids: Discovery of the two-enzyme pathway leading to (-)-sandaracopimaradiene in the fungusBeilstein journal of organic chemistry · 2024Article
Corrections and comments
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Authors and funding
17 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A synthetic biology method based on heterologous biosynthesis coupled with genome mining is a promising approach for increasing the opportunities to rationally access natural product with novel structures and biological activities through total biosynthesis and combinatorial biosynthesis. Here, we demonstrate the advantage of the synthetic biology method to explore biological activity-related chemical space through the comprehensive heterologous biosynthesis of fungal decalin-containing diterpenoid pyrones (DDPs). Genome mining reveals putative DDP biosynthetic gene clusters distributed in five fungal genera. In addition, we design extended DDP pathways by combinatorial biosynthesis. In total, ten DDP pathways, including five native pathways, four extended pathways and one shunt pathway, are heterologously reconstituted in a genetically tractable heterologous host, Aspergillus oryzae, resulting in the production of 22 DDPs, including 15 new analogues. We also demonstrate the advantage of expanding the diversity of DDPs to probe various bioactive molecules through a wide range of biological evaluations.
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