ArticleNature communications2020
Structural dynamics in proteins induced by and probed with X-ray free-electron laser pulses.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 96 citations in OpenAlex.
- Radiation damage in serial femtosecond crystallography studied in hemoglobin.Acta crystallographica. Section D, Structural biology · 2026Article
- Precisely Timed On-Demand Droplet Ejection and Collision in Free Space for Controlled Reaction Initiation.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Aromatic ring flips reveal reshaping of protein dynamics in crystals and complexes.Nature chemistry · 2026Article
- Structural basis of the promiscuity of the unusual Fe(II) and 2-oxoglutarate dependent human aspartate/asparagine-β-hydroxylase.Nature communications · 2026Article
- Article
- Heavy-element damage seeding in proteins under XFEL illumination.Journal of synchrotron radiation · 2025Article
- Calculation of effective pump dose in X-ray-pump/X-ray-probe experiments.Journal of synchrotron radiation · 2025Article
- Impact of gas background on XFEL single-particle imaging.Scientific reports · 2025Article
- On the use of beam precession for serial electron crystallography.Journal of applied crystallography · 2025Article
- Exploring the potential of a bioassembler for protein crystallization in space.NPJ microgravity · 2025Article
- Damage before destruction? X-ray-induced changes in single-pulse serial femtosecond crystallography.IUCrJ · 2025Article
- Compact laser-driven plasma X-ray source for time-resolved diffraction, spectroscopy and imaging experiments at ELI Beamlines.Journal of synchrotron radiation · 2025Article
- Advancing macromolecular structure determination with microsecond X-ray pulses at a 4th generation synchrotron.Communications chemistry · 2025Article
- Effect of crystal-to-detector distance shift on data processing in serial crystallography.PloS one · 2025Article
- Comprehensive characterization of gas dynamic virtual nozzles for x-ray free-electron laser experiments.Structural dynamics (Melville, N.Y.) · 2024Article
- Exploring the dynamics of allostery through multi-dimensional crystallography.Biophysical reviews · 2024Review
- A snapshot love story: what serial crystallography has done and will do for us.Acta crystallographica. Section D, Structural biology · 2024Review
- Theoretical Studies of Anisotropic Melting of Ice Induced by Ultrafast Nonthermal Heating.ACS physical chemistry Au · 2024Article
- Appraising protein conformational changes by resampling time-resolved serial x-ray crystallography data.Structural dynamics (Melville, N.Y.) · 2024Article
- Computational study of diffraction image formation from XFEL irradiated single ribosome molecule.Scientific reports · 2024Article
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Authors and funding
26 authors at 5 institutions in 4 countries.
Funding
Abstract
X-ray free-electron lasers (XFELs) enable crystallographic structure determination beyond the limitations imposed upon synchrotron measurements by radiation damage. The need for very short XFEL pulses is relieved through gating of Bragg diffraction by loss of crystalline order as damage progresses, but not if ionization events are spatially non-uniform due to underlying elemental distributions, as in biological samples. Indeed, correlated movements of iron and sulfur ions were observed in XFEL-irradiated ferredoxin microcrystals using unusually long pulses of 80 fs. Here, we report a femtosecond time-resolved X-ray pump/X-ray probe experiment on protein nanocrystals. We observe changes in the protein backbone and aromatic residues as well as disulfide bridges. Simulations show that the latter's correlated structural dynamics are much slower than expected for the predicted high atomic charge states due to significant impact of ion caging and plasma electron screening. This indicates that dense-environment effects can strongly affect local radiation damage-induced structural dynamics.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.