ArticleDaru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences2020
Validated spectrophotometric method for quantification of total triterpenes in plant matrices.
Article in Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 29 citations in OpenAlex.
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- Bioprospecting for Rhizobacteria with the Ability to Enhance Drought Tolerance inInternational journal of molecular sciences · 2023Article
- Hemolysis by Saponin Is Accelerated at Hypertonic Conditions.Molecules (Basel, Switzerland) · 2023Article
- Improvement of triterpenoid production in mycelia ofHeliyon · 2023Article
- Pullulan/Poly(vinyl alcohol) Hydrogels Loaded withPharmaceutics · 2023Article
- Nanostructured lipid carrier system encapsulating bergamot essential oil within an ionic gel-virgin coconut oil matrix: A promising approach for skin health care.Journal of advanced pharmaceutical technology & researchArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTriterpenes are ubiquitous secondary metabolites present in plants. They can be found in both forms, as genins or conjugated as glycosides. Although distinct analytical methods to quantify these compounds in vegetal tissues are available in the literature, limitations like high cost, complexity on sample preparation, and selectivity are often challenging issues. This study aimed to develop and to validate a simple and rapid spectrophotometric method to detect and quantify total triterpenes in plant matrices.
methodsThe assay was conducted directly into glass tubes using vanillin, acetic acid, and sulphuric acid as reagents, and β-sitosterol as reference standard. The samples were analyzed at 548 nm assessing the quality parameters of selectivity, linearity, precision, accuracy, limit of detection (LOD), limit of quantification (LOQ), and robustness.
resultsThe method was selective, with precision and accuracy varying from 0.56% to 4.98% and 96.63% to 113.87%, respectively. The values of the limit of detection and quantification were 0.042 μg.mL
conclusionThe method was reliable to quantify total triterpenes extracted from Maytenus ilicifolia and Bauhinia holophylla. Graphical abstract.
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