ArticleScience advances2020
Force production of human cytoplasmic dynein is limited by its processivity.
Article in Science advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- Many dynein teams collectively generate high forces during the transport of large organelles.Biophysical journal · 2026Article
- Adaptor-mediated recruitment of three dyneins to dynactin enhances force generation.Nature cell biology · 2026Article
- Measuring molecular forces inside living cells using magnetic tweezers.Biophysical reviews · 2025Review
- Extreme-value analysis in nano-biological systems: applications and implications.Biophysical reviews · 2024Review
- Kinesin-14 HSET and KlpA are non-processive microtubule motors with load-dependent power strokes.Nature communications · 2024Article
- Optical Tweezers Apparatus Based on a Cost-Effective IR Laser-Hardware and Software Description.Sensors (Basel, Switzerland) · 2024Article
- Insect Cell-Based Expression of Cytoskeletal Motor Proteins for Single-Molecule Studies.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Measurements of the Force-Dependent Detachment Rates of Cytoplasmic Dynein from Microtubules.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Article
- Generation of ciliary beating by steady dynein activity: the effects of inter-filament coupling in multi-filament models.Journal of the Royal Society, Interface · 2022Article
- Single-Molecule Studies on the Motion and Force Generation of the Kinesin-3 Motor KIF1A.Methods in molecular biology (Clifton, N.J.) · 2022Article
- On and off controls within dynein-dynactin on native cargoes.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Structure and Mechanics of Dynein Motors.Annual review of biophysics · 2021Review
- Dynein self-organizes while translocating the centrosome in T-cells.Molecular biology of the cell · 2021Article
- Pathogenic mutations in the kinesin-3 motor KIF1A diminish force generation and movement through allosteric mechanisms.The Journal of cell biology · 2021Article
- A highly conserved 3Science advances · 2021Article
- The regulatory function of the AAA4 ATPase domain of cytoplasmic dynein.Nature communications · 2020Article
- Meeting report - NSF-sponsored workshop 'Progress and Prospects of Single-Molecule Force Spectroscopy in Biological and Chemical Sciences'.Journal of cell science · 2020Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Cytoplasmic dynein is a highly complex motor protein that generates forces toward the minus end of microtubules. Using optical tweezers, we demonstrate that the low processivity (ability to take multiple steps before dissociating) of human dynein limits its force generation due to premature microtubule dissociation. Using a high trap stiffness whereby the motor achieves greater force per step, we reveal that the motor's true maximal force ("stall force") is ~2 pN. Furthermore, an average force versus trap stiffness plot yields a hyperbolic curve that plateaus at the stall force. We derive an analytical equation that accurately describes this curve, predicting both stall force and zero-load processivity. This theoretical model describes the behavior of a kinesin motor under low-processivity conditions. Our work clarifies the true stall force and processivity of human dynein and provides a new paradigm for understanding and analyzing molecular motor force generation for weakly processive motors.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.