Evidence map›Paper›PMID 32284628›Full record

ArticleApplied spectroscopy1999

Measurement of the Intensity of Long-Lifetime Luminophores in the Presence of Background Signals Using Phase-Modulation Fluorometry.

Henryk Szmacinski, Joseph R Lakowicz

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied spectroscopy, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Plasmon-controlled fluorescence towards high-sensitivity optical sensing.Advances in biochemical engineering/biotechnology · 2009
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Henryk SzmacinskiDepartment of Molecular Biology and Biochemistry, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, Maryland 21201.
Joseph R LakowiczDepartment of Molecular Biology and Biochemistry, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, Maryland 21201.

Funding

WHO'S WHO IN FLUORESCENCEP41RR008119 · NCRR · UNIVERSITY OF MARYLAND BALTIMORE · PI CHEUNG, HERBERT C · 1993 to 2006
$7.4M
NCRR NIH HHS P41 RR008119
6 · The paper itself

Abstract

We describe a method to correct for background fluorescence and to measure the intensity of long-lifetime probes using phase-modulation fluorometry. The theoretically predicted and simulated data were supported by two experiments. The fractional contribution of background fluorescence in the sample was determined by measurement of phase angle and/or modulation at single modulation frequency. In certain experimental situations, where the mean decay times of the background and the long lifetime probe are widely separated, determination of background signals in the sample does not require a blank sample or information about the nature of intensity decay of the background. Hence, phase-modulation fluorometry can be used to directly determine the intensity of the long-lifetime probe in the presence of an unknown short-lifetime background. We also discuss the effects of ambient light (indefinitely long lifetime) and scattered excitation light (zero lifetime) on phase-modulation measurements.

Indexed as

AutofluorescenceBackgroundFluorescenceFrequency domainMetal–ligand complexPhase modulationSensing

Identifiers

PMID32284628
PMCPMC7153505

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.