ReviewNature methods2020
Scanless two-photon excitation with temporal focusing.
Review in Nature methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
62 citing papers in PubMed.
- CLOK: a chemigenetic multicolor labeling system to visualize neuronal birthdate and circuit integration.Nature neuroscience · 2026Article
- Methods, Trade-offs, and Opportunities in High-speed Optical Microscopy for Neural Voltage imaging.Nature photonics · 2026Article
- Wide-field two-photon random illumination microscopy (2P-RIM).Science advances · 2026Article
- The Importance of Synchrony in the Neural Control of Movement.bioRxiv : the preprint server for biology · 2026Article
- Theoretical analysis of low power synergistic sono-optogenetic control of calcium-dependent synaptic plasticity.Scientific reports · 2026Article
- Grayscale projection two-photon lithography using sub-diffraction motifs for ultrafast and precise nanoscale 3D printing.Nature communications · 2026Article
- STIMscope: centimeter-scale all-optical imaging and patterned optogenetic manipulation at single-cell resolution.bioRxiv : the preprint server for biology · 2026Article
- Scanless temporal focusing enables high-speed three-dimensional quantitative phase microscopy.Research square · 2026Article
- Computational optimization of two-photon holographic stimulation sitesJournal of neural engineering · 2026Article
- Scanless temporal focusing enables high-speed three-dimensional quantitative phase microscopy.bioRxiv : the preprint server for biology · 2026Article
- Active pixel power control for crosstalk-free all-optical neural interrogation.Nature communications · 2026Article
- Article
- Technical development of two-photon optogenetic stimulation and its potential application to brain-machine interfaces.Neurophotonics · 2026Review
- Rapid axially scanned and de-scanned line-scan confocal microscopy with a tunable acoustic gradient index of refraction lens for high-speed volumetricNeurophotonics · 2025Article
- High-throughput synaptic connectivity mapping using in vivo two-photon holographic optogenetics and compressive sensing.Nature neuroscience · 2025Article
- Rapid learning of neural circuitry from holographic ensemble stimulation enabled by model-based compressed sensing.Nature neuroscience · 2025Article
- Optical segmentation-based compressed readout of neuronal voltage dynamics.Nature communications · 2025Article
- Fast photostimulus optimization for holographic control of neural ensemble activitybioRxiv : the preprint server for biology · 2025Article
- Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits.ACS photonics · 2025Review
- Theoretical analysis of low power optogenetic control of synaptic plasticity with subcellular expression of CapChR2 at postsynaptic spine.Scientific reports · 2025Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Temporal focusing, with its ability to focus light in time, enables scanless illumination of large surface areas at the sample with micrometer axial confinement and robust propagation through scattering tissue. In conventional two-photon microscopy, widely used for the investigation of intact tissue in live animals, images are formed by point scanning of a spatially focused pulsed laser beam, resulting in limited temporal resolution of the excitation. Replacing point scanning with temporally focused widefield illumination removes this limitation and represents an important milestone in two-photon microscopy. Temporal focusing uses a diffusive or dispersive optical element placed in a plane conjugate to the objective focal plane to generate position-dependent temporal pulse broadening that enables axially confined multiphoton absorption, without the need for tight spatial focusing. Many techniques have benefitted from temporal focusing, including scanless imaging, super-resolution imaging, photolithography, uncaging of caged neurotransmitters and control of neuronal activity via optogenetics.
Indexed as
Identifiers
32284609What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.