ArticleNucleic acids research2020
Polyphosphate induces the proteolysis of ADP-bound fraction of initiator to inhibit DNA replication initiation upon stress in Escherichia coli.
Article in Nucleic acids research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 39 citations in OpenAlex.
- PolyP inhibits CobB deacetylase triggering a regulatory coupling to prevent DNA replication initiation.Nucleic acids research · 2026Article
- Mechanisms of chromosomal DNA replication in Escherichia coli and Bacillus subtilis.FEMS microbiology reviews · 2026Review
- The chromosomal origin of replication as the basis for the spatio-temporal biology of bacteria.Frontiers in microbiology · 2026Review
- Article
- A novel membrane stress response that blocks chromosomal replication by targeting the DnaA initiator via the ClpP protease.Journal of bacteriology · 2025Article
- Polyphosphate from Lactic Acid Bacteria: A Functional Molecule for Food and Health Applications.Foods (Basel, Switzerland) · 2025Review
- Live tracking of replisomes reveals nutrient-dependent regulation of replication elongation rates in Caulobacter crescentus.Current biology : CB · 2025Article
- Polyphosphate: The "Dark Matter" of Bacterial Chromatin Structure.Molecular microbiology · 2025Review
- Article
- Reentrant DNA shells tune polyphosphate condensate size.Nature communications · 2024Article
- Inorganic polyphosphate and the stringent response coordinately control cell division and cell morphology inbioRxiv : the preprint server for biology · 2024Article
- Polyphosphate kinase regulates LPS structure and polymyxin resistance during starvation in E. coli.PLoS biology · 2024Article
- Polyphosphate kinase deletion increases laboratory productivity in cyanobacteria.Frontiers in plant science · 2024Article
- Cell cycle-coordinated maintenance of theEcoSal Plus · 2023Review
- Advancing evolution: Bacteria break down gene silencer to express horizontally acquired genes.BioEssays : news and reviews in molecular, cellular and developmental biology · 2023Article
- Reentrant DNA shells tune polyphosphate condensate size.bioRxiv : the preprint server for biology · 2023Article
- Inorganic Polyphosphate-Regulator of Cellular Metabolism in Homeostasis and Disease.Biomedicines · 2022Review
- The role of nitrogen-responsive regulators in controlling inorganic polyphosphate synthesis inMicrobiology (Reading, England) · 2022Article
- Link Between Antibiotic Persistence and Antibiotic Resistance in Bacterial Pathogens.Frontiers in cellular and infection microbiology · 2022Review
- Negative feedback for DARS2-Fis complex by ATP-DnaA supports the cell cycle-coordinated regulation for chromosome replication.Nucleic acids research · 2021Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The decision whether to replicate DNA is crucial for cell survival, not only to proliferate in favorable conditions, but also to adopt to environmental changes. When a bacteria encounters stress, e.g. starvation, it launches the stringent response, to arrest cell proliferation and to promote survival. During the stringent response a vast amount of polymer composed of phosphate residues, i.e. inorganic polyphosphate (PolyP) is synthesized from ATP. Despite extensive research on PolyP, we still lack the full understanding of the PolyP role during stress. It is also elusive what is the mechanism of DNA replication initiation arrest in starved Escherichia coli cells. Here, we show that during stringent response PolyP activates Lon protease to degrade selectively the replication initiaton protein DnaA bound to ADP, but not ATP. In contrast to DnaA-ADP, the DnaA-ATP does not interact with PolyP, but binds to dnaA promoter to block dnaA transcription. The systems controlling the ratio of nucleotide states of DnaA continue to convert DnaA-ATP to DnaA-ADP, which is proteolysed by Lon, thereby resulting in the DNA replication initiation arrest. The uncovered regulatory mechanism interlocks the PolyP-dependent protease activation with the ATP/ADP cycle of dual-functioning protein essential for bacterial cell proliferation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.