ArticlePLoS neglected tropical diseases2020
The invasive Asian bush mosquito Aedes japonicus found in the Netherlands can experimentally transmit Zika virus and Usutu virus.
Article in PLoS neglected tropical diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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28 citing papers in PubMed, 1 synthesis or guideline pooled it, 53 citations in OpenAlex.
- Secondary vectors of Zika Virus, a systematic review of laboratory vector competence studies.PLoS neglected tropical diseases · 2023Pooled it
- The global compendium of Aedes japonicus: An Underappreciated Arbovirus Vector of growing concerns.Scientific data · 2026Article
- Invasion dynamics of the disease vector Aedes japonicus in Spain.Scientific reports · 2026Article
- Diversity and persistence of the RNA virome of Philaenus spumarius, the European vector of Xylella fastidiosa.Scientific reports · 2025Article
- The impact of temperature and insect-specific viruses on the transmission of alphaviruses byMicrobiology spectrum · 2025Article
- Ixodes ricinus as potential vector for Usutu virus.PLoS neglected tropical diseases · 2024Article
- The potential role of the Asian bush mosquito Aedes japonicus as spillover vector for West Nile virus in the Netherlands.Parasites & vectors · 2024Article
- Winged Threat on the Offensive: A Literature Review Due to the First Identification ofViruses · 2024Review
- Epidemiology, surveillance and diagnosis of Usutu virus infection in the EU/EEA, 2012 to 2021.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2023Review
- Complete mitogenome sequence ofMitochondrial DNA. Part B, Resources · 2023Article
- Complete genome ofmicroPublication biology · 2023Article
- The virome of the invasive Asian bush mosquitoVirus evolution · 2023Article
- Article
- dynamAedes: a unified modelling framework for invasive Aedes mosquitoes.Parasites & vectors · 2022Article
- Zika vector competence data reveals risks of outbreaks: the contribution of the European ZIKAlliance project.Nature communications · 2022Article
- Emergence of the invasive Asian bush mosquito Aedes (Hulecoeteomyia) japonicus (Theobald, 1901) in the Czech Republic.Parasites & vectors · 2022Article
- A Survey on Native and Invasive Mosquitoes and Other Biting Dipterans in Northern Spain.Acta parasitologica · 2022Article
- CNS endothelial derived extracellular vesicles are biomarkers of active disease in multiple sclerosis.Fluids and barriers of the CNS · 2022Article
- The new invasive mosquito speciesFrontiers in microbiology · 2022Review
- Defining virus-carrier networks that shape the composition of the mosquito core virome of a local ecosystem.Virus evolution · 2022Article
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Authors and funding
15 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Asian bush mosquito Aedes japonicus is invading Europe and was first discovered in Lelystad, the Netherlands in 2013, where it has established a permanent population. In this study, we investigated the vector competence of Ae. japonicus from the Netherlands for the emerging Zika virus (ZIKV) and zoonotic Usutu virus (USUV). ZIKV causes severe congenital microcephaly and Guillain-Barré syndrome in humans. USUV is closely related to West Nile virus, has recently spread throughout Europe and is causing mass mortality of birds. USUV infection in humans can result in clinical manifestations ranging from mild disease to severe neurological impairments. METHODOLOGY/PRINCIPAL
findingsIn our study, field-collected Ae. japonicus females received an infectious blood meal with ZIKV or USUV by droplet feeding. After 14 days at 28°C, 3% of the ZIKV-blood fed mosquitoes and 13% of the USUV-blood fed mosquitoes showed virus-positive saliva, indicating that Ae. japonicus can transmit both viruses. To investigate the effect of the mosquito midgut barrier on virus transmission, female mosquitoes were intrathoracically injected with ZIKV or USUV. Of the injected mosquitoes, 96% (ZIKV) and 88% (USUV) showed virus-positive saliva after 14 days at 28°C. This indicates that ZIKV and USUV can efficiently replicate in Ae. japonicus but that a strong midgut barrier is normally restricting virus dissemination. Small RNA deep sequencing of orally infected mosquitoes confirmed active replication of ZIKV and USUV, as demonstrated by potent small interfering RNA responses against both viruses. Additionally, de novo small RNA assembly revealed the presence of a novel narnavirus in Ae. japonicus. CONCLUSIONS/SIGNIFICANCE: Given that Ae. japonicus can experimentally transmit arthropod-borne viruses (arboviruses) like ZIKV and USUV and is currently expanding its territories, we should consider this mosquito as a potential vector for arboviral diseases in Europe.
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