ArticleMarine biotechnology (New York, N.Y.)2020
Sex-Specific Transcriptome Sequencing of Zoea I Larvae and Identification of Sex-Linked Genes Using Bulked Segregant Analysis in Pacific White Shrimp Litopenaeus vannamei.
Article in Marine biotechnology (New York, N.Y.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 42 citations in OpenAlex.
- Sex reversal induced by 17β-estradiol may be achieved by regulating the neuroendocrine system of the Pacific white shrimp Penaeus vannamei.BMC genomics · 2025Article
- Nanopore-Based Sequencing of the Full-Length Transcriptome of Male and Female Cleavage-Stage Embryos of the Chinese Mitten Crab (International journal of molecular sciences · 2024Article
- High-quality chromosome-level genome assembly of femaleHeliyon · 2024Article
- A high-density linkage map and sex-determination loci in Pacific white shrimp (Litopenaeus vannamei).BMC genomics · 2024Article
- Monosex Populations of the Giant Freshwater PrawnInternational journal of molecular sciences · 2023Review
- Differentially expressed genes in the testes from early to mature development of banana shrimp (Fenneropenaeus merguiensis).PloS one · 2023Article
- Allelically and Differentially Expressed Genes After Infection of Edwardsiella ictaluri in Channel Catfish as Determined by Bulk Segregant RNA-Seq.Marine biotechnology (New York, N.Y.) · 2022Article
- Sex-Biased Gene Expression and Isoform Profile of Brine ShrimpAnimals : an open access journal from MDPI · 2021Article
- Pomc Plays an Important Role in Sexual Size Dimorphism in Tilapia.Marine biotechnology (New York, N.Y.) · 2021Article
- Review
- Article
- Updated Genome Assembly of Bighead Carp (Frontiers in genetics · 2021Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
In order to learn the sex determination and differentiation process in shrimp, we performed sex-specific transcriptome sequencing of the Zoea I larvae in Litopenaeus vannamei (L. vannamei) using a DNA/RNA co-extraction method. The sex-specific genes and the sex-linked SNPs were identified. De novo assembly of all the clean reads generated 41,270 unigenes with an average length of 1026 bp and an N50 of 1922 bp. A total of 60 differentially expressed genes (DEGs) between females and males at Zoea I stage were obtained, in which 41 unigenes had annotations. DEGs that might be related to sex development in L. vannamei were analyzed. Besides, paralogous genes of some known sex-related genes in model organisms such as sex-lethal (Sxl) showed no sex-biased difference, which indicated they may not play roles in sex development at Zoea I stage. Candidate sex-linked SNPs were validated in six populations of L. vannamei, and SNPs in Unigene0020898 and Unigene0020336 were fully linked to genders, suggesting the two genes located in the sex-determining region of L. vannamei. Our report provided the sex-specific gene expression in early developmental stage of L. vannamei, and found some sex-biased genes that may participate in the sex development process. We also detected two sex-linked genes which may be located in the sex determination region of L. vannamei through bulked segregant analysis, and the SNPs on these genes also provided a method for genetic sex identification at transcriptional level. Our findings will facilitate further researches on molecular mechanism of sex determination and differentiation in shrimps.
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