Evidence map›Paper›PMID 32279222›Full record

ArticleFood and environmental virology2020

Clinical and Environmental Surveillance of Rotavirus Common Genotypes Showed High Prevalence of Common P Genotypes in Egypt.

Waled M El-Senousy, Amel S M Abu Senna, Nabil A Mohsen, Seham F Hasan, Nagwa M Sidkey

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Food and environmental virology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed, 3 pooled it
0.5field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 3 syntheses or guidelines pooled it, 34 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Pooled it
  4. Review
  5. Review
  6. Article
  7. Review
  8. Review
  9. Article
  10. Viral Eco-Genomic Tools: Development and Implementation for Aquatic Biomonitoring.International journal of environmental research and public health · 2022
    Review
  11. Temporal variations of human and animalFrontiers in microbiology · 2022
    Article
  12. Article
  13. Detection and evaluation of rotavirus surveillance methods as viral indicator in the aquatic environments.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021
    Review
  14. Review
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Waled M El-SenousyEnvironmental Virology Lab., Water Pollution Research Department, Environmental Research Division and Food-Borne Viruses Group, Centre of Excellence for Advanced Sciences, National Research Centre (NRC), 33 El-Buhouth st., Dokki, P.O. 12622, Giza, Egypt. waledmorsy@hotmail.com.ORCID 0000-0003-0649-0728
Amel S M Abu SennaBotany and Microbiology Department, Faculty of Science for Girls, Al-Azhar University, Yossuf Abbas st., Nasr city, P.O. 11754, Cairo, Egypt.
Nabil A MohsenPediatrics Department, Kasr Al Ainy School of Medicine, Cairo University, Kasr Al Ainy st, P.O. 11562, Cairo, Egypt.
Seham F HasanBotany and Microbiology Department, Faculty of Science for Girls, Al-Azhar University, Yossuf Abbas st., Nasr city, P.O. 11754, Cairo, Egypt.
Nagwa M SidkeyBotany and Microbiology Department, Faculty of Science for Girls, Al-Azhar University, Yossuf Abbas st., Nasr city, P.O. 11754, Cairo, Egypt.
Al-Azhar University · EGCairo University · EGNational Research Centre · EG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The objective of this study was to compare the prevalence of human rotavirus group A common G and P genotypes in human Egyptian stool specimens and raw sewage samples to determine the most common genotypes for future vaccine development. From 1026 stool specimens of children with acute diarrhea and using nested RT-PCR, 250 samples (24.37%) were positive for human rotavirus group A. Using multiplex RT-PCR, rotavirus common P and G genotypes were detected as 89.20% and 46.40% of the positive clinical specimens respectively. This low percentage of common G genotypes frequency may affect the efficiency of the available live attenuated oral rotavirus vaccines [Rotarix® (human rotavirus G1P[8]) and RotaTeq® (reassortant bovine-human rotavirus G1-4P[5] and G6P[8])], however the percentage of clinical specimens which were negative for common G genotypes but positive for P[8] genotype was 12.00%. From 24 positive raw sewage samples for rotavirus group A VP6 collected from Zenin and El-Gabal El-Asfar wastewater treatment plants (WWTPs), 21 samples (87.50%) were typeable for common P genotypes while 13 samples (54.17%) were typeable for common G genotypes. Phylogenetic analysis of a VP8 partial gene of 45 P-typeable clinical isolates and 20 P-typeable raw sewage samples showed high similarity to reference strains and the majority of mutations were silent and showed lower to non-significant similarity with the two vaccine strains. This finding is useful for determining the most common antigens required for future vaccine development.

Indexed as

ChildChild, PreschoolDiarrheaEgyptEnvironmental MonitoringFemaleGenotypeHumansInfantInfant, NewbornMaleMolecular EpidemiologyPhylogenyPrevalenceRecombination, GeneticRotavirusSewageCommon P and G genotypesDiarrheaHuman rotavirus group ANon-silent mutationSurveillance

Identifiers

PMID32279222
PMCPMC7224034
OpenAlexW3015466724

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.