Evidence map›Paper›PMID 32279210›Full record

ArticleMolecular biology reports2020

Overexpression of GmSUMO2 gene confers increased abscisic acid sensitivity in transgenic soybean hairy roots.

Jingsong Guo, Sibo Wang, Guixin Wang, Runfeng Lu, Yuxin Wang, Yushuang Guo, Wei Ji

Abstract read
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In one paragraph

Article in Molecular biology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Jingsong GuoCollege of Life Science, Northeast Agricultural University, Harbin, 150030, China.
Sibo WangCollege of Life Science, Northeast Agricultural University, Harbin, 150030, China.
Guixin WangCollege of Life Science, Northeast Agricultural University, Harbin, 150030, China.
Runfeng LuCollege of Life Science, Northeast Agricultural University, Harbin, 150030, China.
Yuxin WangCollege of Life Science, Northeast Agricultural University, Harbin, 150030, China.
Yushuang GuoKey Laboratory of Molecular Genetics, China National Tobacco Corporation, Guizhou Institute of Tobacco Science, Guiyang, 550083, China.
Wei JiCollege of Life Science, Northeast Agricultural University, Harbin, 150030, China. iwei_ji@neau.edu.cn.ORCID http://orcid.org/0000-0003-2079-4736
Northeast Agricultural University · CNChina Tobacco · CN

Funding

National Natural Science Foundation of China 31971832Natural Science Foundation of Heilongjiang Province of China YQ2019C006
6 · The paper itself

Abstract

Small ubiquitin-like modifier (SUMO) participates in post-translational modification of various target proteins. SUMOylation is an important molecular regulatory mechanism for plants to respond to abiotic stress. In the present study, GmSUMO2 gene was isolated from soybean seedlings for further study because of the highest expression level among these six SUMO genes in soybean. qRT-PCR results showed that GmSUMO2 gene were detected in root, leaf, cotyledon, seed root, flower, pod and seed, with the highest transcription level in cotyledon. Moreover, GmSUMO2 gene was transcriptionally regulated by 200 mM NaCl, 42 °C, 25 μM abscisic acid (ABA) and 20% PEG6000 during the 24 h period of treatment. Besides, western blot analysis using AtSUMO1 antibody indicated that the free SUMO levels and SUMOylation dynamics were regulated by ABA stimulus. Functional analysis indicated that overexpression of GmSUMO2 gene in soybean hairy roots accentuated the sensitivity to exogenous ABA. Furthermore, the expression levels of ABI3, ABI5, SnRK1.1 and SnRK1.2 were differentially regulated by GmSUMO2 in transgenic soybean hairy roots. Overall, these results provided a preliminary understanding of molecular characterization, expression and function of GmSUMO2 in soybean.

Indexed as

Abscisic AcidBasic-Leucine Zipper Transcription FactorsGene Expression Regulation, PlantGlycine maxPlant ProteinsPlant RootsPlants, Genetically ModifiedProtein Serine-Threonine KinasesSeedlingsSeedsSignal TransductionSmall Ubiquitin-Related Modifier ProteinsStress, PhysiologicalAbscisic AcidBasic-Leucine Zipper Transcription FactorsPlant ProteinsProtein Serine-Threonine KinasesSmall Ubiquitin-Related Modifier ProteinsABAGmSUMO2Transcriptional regulationTransgenic soybean hairy roots

Identifiers

PMID32279210
OpenAlexW3015232417

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.