Evidence map›Paper›PMID 32279137›Full record

ArticleJournal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry2020

The effect of an engineered ATCUN motif on the structure and biophysical properties of the SH3 domain of c-Src tyrosine kinase.

Marina Plaza-Garrido, Mª Carmen Salinas-García, José C Martínez, Ana Cámara-Artigas

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Article in Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.2field-weighted citation impact, top 61% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Understanding domain swapping in the c-Src SH3 domain through hinge-loop mutagenesis.Acta crystallographica. Section D, Structural biology · 2025
    Article
  2. Nickel binding to c-Src SH3 domain facilitates crystallization.bioRxiv : the preprint server for biology · 2025
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Marina Plaza-GarridoDepartment of Chemistry and Physics, University of Almería, Agrifood Campus of International Excellence ceiA3 and CIAMBITAL, 04120, Almería, Spain.
Mª Carmen Salinas-GarcíaDepartment of Chemistry and Physics, University of Almería, Agrifood Campus of International Excellence ceiA3 and CIAMBITAL, 04120, Almería, Spain.
José C MartínezDepartment of Physical Chemistry and Institute of Biotechnology, Faculty of Sciences, University of Granada, Granada, Spain.
Ana Cámara-ArtigasDepartment of Chemistry and Physics, University of Almería, Agrifood Campus of International Excellence ceiA3 and CIAMBITAL, 04120, Almería, Spain. acamara@ual.es.
University of Almería · ESUniversidad de Granada · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Metal binding to sites engineered in proteins can provide an increase in their stability and facilitate new functions. Besides the sites introduced in purpose, sometimes they are present accidentally as a consequence of the expression system used to produce the protein. This happens with the copper- and nickel-binding (ATCUN) motif generated by the amino-terminal residues Gly-Ser-His. This ATCUN motif is fortuitously present in many proteins, but how it affects the structural and biophysical characterization of the proteins has not been studied. In this work, we have compared the structure and biophysical properties of a small modular domain, the SH3 domain of the c-Src tyrosine kinase, cloned with and without an ATCUN motif at the N terminus. At pH 7.0, the SH3 domain with the ATCUN motif binds nickel with a binding constant K

Indexed as

Protein EngineeringBinding SitesCopperCSK Tyrosine-Protein KinaseHumansNickelProtein ConformationCopperCSK protein, humanCSK Tyrosine-Protein KinaseNickelATCUN motifCircular dichroismCrystal structureFluorescenceNickel bindingSH3 domain

Identifiers

PMID32279137
OpenAlexW3016103688

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.