ArticleKnee surgery, sports traumatology, arthroscopy : official journal of the ESSKA2021
Excellent histological results in terms of articular cartilage regeneration after spheroid-based autologous chondrocyte implantation (ACI).
Article in Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.
- Role of Matrix-Associated Autologous Chondrocyte Implantation with Spheroids in the Treatment of Large Chondral Defects in the Knee: A Systematic Review.International journal of molecular sciences · 2021Pooled it
- Article
- Clinical Effectiveness and Magnetic Resonance Imaging-Based Endurability of Matrix-Associated Autologous Chondrocyte Implantation with an Autologous Periosteal Flap for Articular Cartilage Defects of the Knee Joint.Journal of clinical medicine · 2026Article
- Review
- Autologous bone grafting combined with spheroid-based matrix-induced autologous chondrocyte implantation for osteochondral defects of the knee: Good clinical outcomes alongside abnormal postoperative gait patterns.Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA · 2025Article
- The transepicondylar distance is a reliable and easily measured parameter for estimating femoral cartilage surface area using MRI.Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA · 2025Article
- Autologous minced cartilage repair for chondral and osteochondral lesions of the knee joint demonstrates good postoperative outcomes and low reoperation rates at minimum five-year follow-up.Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA · 2023Article
- Knee Cartilage Lesion Management-Current Trends in Clinical Practice.Journal of clinical medicine · 2023Review
- Spheroid Culture System, a Promising Method for Chondrogenic Differentiation of Dental Mesenchymal Stem Cells.Biomedicines · 2023Article
- Therapeutic strategies of three-dimensional stem cell spheroids and organoids for tissue repair and regeneration.Bioactive materials · 2023Review
- Recent Developments and Current Applications of Organic Nanomaterials in Cartilage Repair.Bioengineering (Basel, Switzerland) · 2022Review
- Safety and Efficacy of Matrix-Associated Autologous Chondrocyte Implantation With Spheroids for Patellofemoral or Tibiofemoral Defects: A 5-Year Follow-up of a Phase 2, Dose-Confirmation Trial.Orthopaedic journal of sports medicine · 2022Article
- Repairing Cartilage with Processed Chondrocyte Constructs: A 6-Month Study Using a Porcine Model.Cartilage · 2021Article
- Crosstalk Between Mesenchymal Stromal Cells and Chondrocytes: The Hidden Therapeutic Potential for Cartilage Regeneration.Stem cell reviews and reports · 2021Review
- Therapies with CCL25 require controlled release via microparticles to avoid strong inflammatory reactions.Journal of nanobiotechnology · 2021Article
- Early Efficacy of Type I Collagen-Based Matrix-Assisted Autologous Chondrocyte Transplantation for the Treatment of Articular Cartilage Lesions.Frontiers in bioengineering and biotechnology · 2021Article
- Herbal Remedies as Potential in Cartilage Tissue Engineering: An Overview of New Therapeutic Approaches and Strategies.Molecules (Basel, Switzerland) · 2020Review
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Authors and funding
7 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTraumatic lesions of articular cartilage represent a crucial risk factor for osteoarthritis. Even if several strategies exist to treat such damages, the optimal solution has not yet been found. A new strategy represents the scaffold-free spheroid-based autologous chondrocyte transplantation. In this method, spheroids of chondrocytes are synthesized after chondrocyte isolation and expansion, followed by the implantation in a second intervention.
methodsFine Jamshidi-needle biopsies from five patients (one from each patient, Ø 2 mm) treated with a spheroid-based autologous chondrocyte implantation (ACI) after traumatic lesions of the articular cartilage of the knee were analysed histologically and immunohistologically for collagen II, collagen X and aggrecan expression. The indication for a second look arthroscopy was given by arthrofibrosis or meniscus-lesions, respectively. The time between ACI and second-look arthroscopy ranged between 6 and 16 months.
resultsIn all patients, the histological examinations revealed an avascular cartilage tissue with a homogenic extracellular matrix. The subchondral bone neither showed bleeding, necrosis nor hypertrophy. A homogenous alcian blue staining indicated high amounts of mucopolysaccharides and glycosaminoglycans. Collagen II staining was highly positive, whereas collagen X staining was negative in every patient, ruling out hypertrophic chondrocyte differentiation. In addition, intense aggrecan staining indicated a strong expression of this extracellular matrix component.
conclusionThe present case series represents the first histological and immunohistological analyses of spheroid-based ACI in humans. Spheroid-based ACI revealed excellent histological results regarding the regeneration of hyaline articular cartilage. These results indicate that spheroid based ACI is a promising strategy for treating traumatic lesions of the articular cartilage of the knee.
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