ArticleScientific reports2020
Bovine Sperm Sexing Alters Sperm Morphokinetics and Subsequent Early Embryonic Development.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- The reproductive success of bovine sperm after sex-sorting: a meta-analysis.Scientific reports · 2021Pooled it
- Impact of Sorting and Catch Media on Porcine Sperm Motility, Capacitation, and Viability.Andrology · 2026Article
- Effects of Fluorescence-Activated Cell Sorting on Boar Sperm Motility Subpopulations and In Vitro Embryo Development.Molecular reproduction and development · 2026Article
- Enhancement of Y-bearing sperm enrichment in swamp buffalo using a TLR7/8 agonist (R848) combined with discontinuous Percoll density gradient centrifugation.Veterinary world · 2026Article
- Melatonin Supplementation in Sex-Sorted Nili-Ravi Buffalo Semen: Effect on Sperm Quality, Subsequent in Vitro Embryo Development, and Pregnancy Outcomes.Antioxidants (Basel, Switzerland) · 2026Article
- Two decades of sperm sex-sorting in animals: A comprehensive systematic review and meta-analysis of techniques, effectiveness, and global research trends (2005-2025).Veterinary world · 2025Review
- Association between sex and the developmental morphokinetics of in vitro derived bovine embryos.Scientific reports · 2025Article
- Improved Pregnancy Outcomes in Beef Heifers Through Delayed Insemination of Sexed Semen.Reproduction in domestic animals = Zuchthygiene · 2025Article
- Using Cholesterol-Loaded Cyclodextrin to Improve Cryo-Survivability and Reduce Capacitation-Like Changes in Gender-Ablated Jersey Semen.Animals : an open access journal from MDPI · 2025Article
- A review of sustainable cattle genetic improvement in the Peruvian Highlands.Veterinary and animal science · 2025Review
- Paternal Effects in Mammalian Reproduction: Functional, Environmental, and Clinical Relevance of Sperm Components in Early Embryos and Beyond.Molecular reproduction and development · 2025Review
- Assessment of Sperm Binding Capacity in the Reservoir Using an In Vitro Model of Oviduct Epithelial Spheroids in Cattle.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Fertilization rate of crossbreeding cattle using sexing and conventional semen in different seasons in South Papua.Journal of advanced veterinary and animal research · 2024Article
- Environmental Stress-Induced Alterations in Embryo Developmental Morphokinetics.Journal of xenobiotics · 2024Review
- Assessing the Relationship between proAKAP4 Level and Longevity of Sexed Sperm Quality after Thawing.Veterinary sciences · 2024Article
- Comparative developmental competence ofVeterinary world · 2024Article
- Application of imaging and spectroscopy techniques for grading of bovine embryos - a review.Frontiers in veterinary science · 2024Review
- Pre-implantation development of cattle embryos produced from fresh bull semen enriched for X- chromosome-bearing spermatozoa using a monoclonal antibody.Veterinary research communications · 2023Article
- Sorted Bulls' X-Chromosome-Bearing Spermatozoa Show Increased GAPDHS Activity Correlating with Motility.Genes · 2023Article
- Sperm-oviduct interactions: Key factors for sperm survival and maintenance of sperm fertilizing capacity.Andrology · 2022Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In artificial insemination the use of sex-sorted bovine sperm results in reduced conception, the causes of which are only partly understood. Therefore, we set out to investigate the effects of sexing on bovine sperm function and early embryonic development. Computer-assisted semen analysis (CASA) of sperm of the same bulls (n = 5), before and after sexing, demonstrated significantly reduced fast (A) and slow (B) progressively motile sperm (p < 0.05) after sexing. Sexed-sperm also revealed significantly less hyperactivated sperm (p < 0.05). As shown by time-lapse videomicroscopy of in vitro produced embryos (n = 360), embryos derived from sexed-sperm displayed significantly increased incidences of arrest at the 4-cell stage (p < 0.05). The relative risk for shrinkage/fusion of blastomeres with subsequent lysis was 1.71 times higher in the embryos derived from sexed-sperm as compared to conventional embryos (p < 0.05) resulting in significantly reduced blastocyst rates (p < 0.001). The relative risk for cleavage was 2.36 times lower in the embryos derived from sex-sorted sperm (p < 0.001). Additionally, sexed-sperm-derived embryos showed reduced survival times (hazard ratio HR = 1.54, p < 0.001) which were bull dependent (p < 0.001). However, the percentage of apoptotic cells was similar to conventional embryos. Furthermore, embryos derived from sexed-sperm were found to reach developmental stages at similar timings as conventional embryos. Our results suggest that reduced conception rates after sexing are due to altered sperm morphokinetics, decreasing the chance of sperm to reach and fertilise the oocyte, and aberrant early embryonic development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.